دورية أكاديمية
Exploration of risk analysis and elimination methods for a Cr(VI)-removal recombinant strain through a biosafety assessment in mice.
| العنوان: | Exploration of risk analysis and elimination methods for a Cr(VI)-removal recombinant strain through a biosafety assessment in mice. |
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| المؤلفون: | You W; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., An Q; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Guo D; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Huang Z; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Guo L; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Chen Z; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Xu H; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Wang G; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Weng Y; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Ma Z; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Chen X; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China., Hong F; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, People's Republic of China., Zhao R; State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, No. 4221-117 South Xiang'an Road, Xiang'an District, Xiamen 361102, Fujian, People's Republic of China. Electronic address: zhaoran@xmu.edu.cn. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 Feb 20; Vol. 912, pp. 168743. Date of Electronic Publication: 2023 Nov 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Containment of Biohazards* , Disinfectants*/toxicity, Mice ; Animals ; Biodegradation, Environmental ; Chromium/analysis ; Risk Assessment |
| مستخلص: | Though recombinant strains are increasingly recognized for their potential in heavy metal remediation, few studies have evaluated their safety. Moreover, biosafety assessments of fecal-oral pathway exposure at country as well as global level have seldom analyzed the health risks of exposure to microorganisms from a microscopic perspective. The present study aimed to predict the long-term toxic effects of recombinant strains by conducting a subacute toxicity test on the chromium-removal recombinant strain 3458 and analyzing the gut microbiome. The available disinfection methods were also evaluated. The results showed that strain 3458 induced liver damage and affected renal function and lipid metabolism at 1.0 × 10 11 CFU/mL, which may be induced by its carrier strain, pET-28a. Strain 3458 poses the risk of increasing the number of pathogenic bacteria under prolonged exposure. When 500 mg L -1 chlorine-containing disinfectant or 250 mg L -1 chlorine dioxide disinfectant was added for 30 min, the sterilization rate exceeded 99.9 %. These findings suggest that existing wastewater disinfection methods can effectively sterilize strain 3458, ensuring its application value. The present study can serve a reference for the biosafety evaluation of the recombinant strain through exposure to the digestive tract and its feasibility for application in environmental pollution remediation. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that might influence the work described in this paper. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bioremediation; Biosafety evaluation; Hexavalent chromium; Recombinant strain |
| المشرفين على المادة: | 18540-29-9 (chromium hexavalent ion) 0R0008Q3JB (Chromium) 0 (Disinfectants) |
| تواريخ الأحداث: | Date Created: 20231125 Date Completed: 20240118 Latest Revision: 20240118 |
| رمز التحديث: | 20240118 |
| DOI: | 10.1016/j.scitotenv.2023.168743 |
| PMID: | 38007124 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
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| DOI: | 10.1016/j.scitotenv.2023.168743 |