دورية أكاديمية
EHD1 impaired decidualization of endometrial stromal cells in recurrent implantation failure: role of SENP1 in modulating progesterone receptor signalling†.
| العنوان: | EHD1 impaired decidualization of endometrial stromal cells in recurrent implantation failure: role of SENP1 in modulating progesterone receptor signalling†. |
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| المؤلفون: | Zhang H; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Kong L; Nanjing Vocational Health College, Nanjing, China., Cao Z; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Zhu Y; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China., Jiang Y; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China., Wang X; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Jiang R; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Liu Y; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Zhou J; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Kang Y; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Zhen X; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Kong N; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China., Wu M; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China., Yan G; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China., Sun H; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.; Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, China. |
| المصدر: | Biology of reproduction [Biol Reprod] 2024 Mar 13; Vol. 110 (3), pp. 536-547. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Champaign, Ill. : Society for the Study of Reproduction |
| مواضيع طبية MeSH: | Progesterone*/pharmacology , Progesterone*/metabolism , Decidua*/metabolism, Humans ; Female ; Receptors, Progesterone/genetics ; Receptors, Progesterone/metabolism ; Endometrium/metabolism ; Stromal Cells/metabolism ; Vesicular Transport Proteins/metabolism ; Cysteine Endopeptidases |
| مستخلص: | Recurrent implantation failure (RIF) patients exhibit poor endometrial receptivity and abnormal decidualization with reduced effectiveness and exposure to progesterone, which is an intractable clinical problem. However, the associated molecular mechanisms remain elusive. We found that EH domain containing 1 (EHD1) expression was abnormally elevated in RIF and linked to aberrant endometrial decidualization. Here we show that EHD1 overexpressed in human endometrial stromal cells significantly inhibited progesterone receptor (PGR) transcriptional activity and the responsiveness to progesterone. No significant changes were observed in PGR mRNA levels, while a significant decrease in progesterone receptor B (PRB) protein level. Indeed, EHD1 binds to the PRB protein, with the K388 site crucial for this interaction. Overexpression of EHD1 promotes the SUMOylation and ubiquitination of PRB, leading to the degradation of the PRB protein. Supplementation with the de-SUMOylated protease SENP1 ameliorated EHD1-repressed PRB transcriptional activity. To establish a functional link between EHD1 and the PGR signalling pathway, sg-EHD1 were utilized to suppress EHD1 expression in HESCs from RIF patients. A significant increase in the expression of prolactin and insulin-like growth factor-binding protein 1 was detected by interfering with the EHD1. In conclusion, we demonstrated that abnormally high expression of EHD1 in endometrial stromal cells attenuated the activity of PRB associated with progesterone resistance in a subset of women with RIF. (© The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 82171653 National Natural Science Foundation of China; BK20190051 Natural Science Foundation of Jiangsu Province |
| فهرسة مساهمة: | Keywords: EHD1; SENP1; decidualization; progesterone receptor; recurrent implantation failure (RIF) |
| المشرفين على المادة: | 4G7DS2Q64Y (Progesterone) 0 (Receptors, Progesterone) 0 (EHD1 protein, human) 0 (Vesicular Transport Proteins) EC 3.4.- (SENP1 protein, human) EC 3.4.22.- (Cysteine Endopeptidases) |
| تواريخ الأحداث: | Date Created: 20231127 Date Completed: 20240318 Latest Revision: 20240318 |
| رمز التحديث: | 20240318 |
| DOI: | 10.1093/biolre/ioad161 |
| PMID: | 38011671 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1529-7268 |
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| DOI: | 10.1093/biolre/ioad161 |