دورية أكاديمية
Chemical composition, antifungal, and anti-virulence action of the stem bark of Hancornia speciosa Gomes (Apocynaceae) against Candida spp.
| العنوان: | Chemical composition, antifungal, and anti-virulence action of the stem bark of Hancornia speciosa Gomes (Apocynaceae) against Candida spp. |
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| المؤلفون: | Silva VBD; Department of Botany, Universidade Federal de Pernambuco - UFPE, Rua Professor Moraes Rego, s/n, Recife, Pernambuco, 50.670-901, Brazil. Electronic address: viviane.silva@urca.br., Almeida-Bezerra JW; Department of Botany, Universidade Federal de Pernambuco - UFPE, Rua Professor Moraes Rego, s/n, Recife, Pernambuco, 50.670-901, Brazil., Novais MHG; Department of Biological Sciences, Universidade Regional do Cariri - URCA, Rua Cel. Antônio Luiz, 1161, Crato, Ceará, 63.105-000, Brazil., Farias NS; Department of Biological Sciences, Universidade Regional do Cariri - URCA, Rua Cel. Antônio Luiz, 1161, Crato, Ceará, 63.105-000, Brazil., Coelho JJ; Animal Science Department, Universidade Estadual do Maranhão - UEMA, São Luís, Maranhão, Brazil., Ribeiro PRV; Multi-User Natural Products Chemistry Laboratory - LMQPN, Embrapa Agroindustria Tropical, Rua Dra. Sara Mesquita, 2270, Fortaleza, Ceará, 60511-110, Brazil., Canuto KM; Multi-User Natural Products Chemistry Laboratory - LMQPN, Embrapa Agroindustria Tropical, Rua Dra. Sara Mesquita, 2270, Fortaleza, Ceará, 60511-110, Brazil., Coutinho HDM; Department of Biological Chemistry, Universidade Regional do Cariri - URCA, Rua Cel. Antônio Luiz, 1161, Crato, Ceará, 63.105-000, Brazil., Morais-Braga MFB; Department of Biological Sciences, Universidade Regional do Cariri - URCA, Rua Cel. Antônio Luiz, 1161, Crato, Ceará, 63.105-000, Brazil., Oliveira AFM; Department of Botany, Universidade Federal de Pernambuco - UFPE, Rua Professor Moraes Rego, s/n, Recife, Pernambuco, 50.670-901, Brazil. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2024 Mar 01; Vol. 321, pp. 117506. Date of Electronic Publication: 2023 Nov 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Apocynaceae*/chemistry , Candidiasis* , Communicable Diseases*, Antifungal Agents/pharmacology ; Antifungal Agents/chemistry ; Candida ; Fluconazole/pharmacology ; Virulence ; Chromatography, Liquid ; Plant Bark/chemistry ; Anthocyanins/pharmacology ; Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Tandem Mass Spectrometry ; Candida albicans ; Phytochemicals/analysis ; Microbial Sensitivity Tests |
| مستخلص: | Ethnopharmacological Relevance: Hancornia speciosa Gomes is a fruit and medicinal species used for treating infectious diseases of the genitourinary system. However, its mechanism of action against microbes is still not fully understood. Infections in the genitourinary system caused by Candida spp. are associated with its fungal resistance and pathogenicity. New plant-derived compounds are an alternative to fight these Candida infections. Aim of the Study: The objective of this study was to evaluate the anti-Candida effects of extracts of the stem bark of H. speciosa. This research investigated the chemical composition of sulfuric ether (EEHS) and methanolic (MEHS) extracts, their drug-modifying action on fluconazole, and their anti-virulence action on the morphological transition of Candida species. Materials and Methods: The extracts (EEHS and MEHS) of the stem bark of H. speciosa were chemically characterized via qualitative phytochemical screening and by liquid chromatography coupled with mass spectrometry (UPLC-MS-ESI-QTOF). The extracts were evaluated regarding their antifungal effects and fluconazole-modifying activity against Candida albicans, Candida krusei, and Candida tropicalis using the broth microdilution method. Additionally, the study evaluated the inhibition of fungal virulence in Candida species through morphological transition assays. Results: The phytochemical screening revealed the presence of anthocyanidins, anthocyanins, aurones, catechins, chalcones, flavones, flavonols, flavanones, leucoanthocyanidins, tannins (condensed and pyrogallic), and xanthones in both extracts of the stem bark of H. speciosa. The UPLC-MS-ESI-QTOF analysis identified the same compounds in both extracts, predominating phenolic compounds. Some compounds were first time recorded in this species: gluconic acid, cinchonain IIb, cinchonain Ib isomer, and lariciresinol hexoside isomers. Most of the intrinsic antifungal activity was observed for the MEHS against C. krusei (IC Conclusion: The stem bark of H. speciosa is a rich source of bioactive compounds, especially phenolic. Phenolic compounds can have important roles in fighting infectious diseases of the genitourinary system, such as candidiasis. The extracts of H. speciosa improved the action of the drug fluconazole against Candida species, inhibited hyphae appearance, and reduced pseudohyphae formation. The results of this study can support the development of new therapeutics against resistant strains of Candida. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cinchonain; Ethnomedicine; Flavonoids; Hyphae; Phenolic compounds |
| المشرفين على المادة: | 0 (Antifungal Agents) 8VZV102JFY (Fluconazole) 0 (Anthocyanins) 0 (Plant Extracts) 0 (Phytochemicals) |
| تواريخ الأحداث: | Date Created: 20231128 Date Completed: 20240101 Latest Revision: 20240101 |
| رمز التحديث: | 20240101 |
| DOI: | 10.1016/j.jep.2023.117506 |
| PMID: | 38012976 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2023.117506 |