دورية أكاديمية
Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX.
| العنوان: | Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX. |
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| المؤلفون: | Williamson LJ; School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom., Galchenkova M; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Best HL; School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom., Bean RJ; European XFEL GmbH, Schenefeld 22869, Germany., Munke A; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Awel S; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Pena G; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Knoska J; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Schubert R; European XFEL GmbH, Schenefeld 22869, Germany., Dörner K; European XFEL GmbH, Schenefeld 22869, Germany., Park HW; Department of Biological Sciences, California Baptist University, Riverside, CA 92504., Bideshi DK; Department of Biological Sciences, California Baptist University, Riverside, CA 92504., Henkel A; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Kremling V; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Klopprogge B; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Lloyd-Evans E; School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom., Young MT; School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom., Valerio J; European XFEL GmbH, Schenefeld 22869, Germany., Kloos M; European XFEL GmbH, Schenefeld 22869, Germany., Sikorski M; European XFEL GmbH, Schenefeld 22869, Germany., Mills G; European XFEL GmbH, Schenefeld 22869, Germany., Bielecki J; European XFEL GmbH, Schenefeld 22869, Germany., Kirkwood H; European XFEL GmbH, Schenefeld 22869, Germany., Kim C; European XFEL GmbH, Schenefeld 22869, Germany., de Wijn R; European XFEL GmbH, Schenefeld 22869, Germany., Lorenzen K; European XFEL GmbH, Schenefeld 22869, Germany., Xavier PL; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany.; Max-Planck Institute for the Structure and Dynamics of Matter, Hamburg 22761, Germany., Rahmani Mashhour A; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Gelisio L; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Yefanov O; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany., Mancuso AP; European XFEL GmbH, Schenefeld 22869, Germany.; Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia., Federici BA; Department of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521., Chapman HN; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany.; Centre for Ultrafast Imaging, Universität Hamburg, Hamburg 22761, Germany.; Department of Physics, Universität Hamburg, Hamburg 22761, Germany., Crickmore N; School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom., Rizkallah PJ; School of Medicine, Cardiff University, Cardiff CF14 4XN, United Kingdom., Berry C; School of Biosciences, Cardiff University, Cardiff CF10 3AX, United Kingdom., Oberthür D; Center for Free Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Dec 05; Vol. 120 (49), pp. e2203241120. Date of Electronic Publication: 2023 Nov 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Pesticides* , Bacillus* , Bacillaceae*/chemistry , Bacillaceae*/metabolism , Culex*, Animals ; Mosquito Control ; Larva/metabolism |
| مستخلص: | The Lysinibacillus sphaericus proteins Tpp49Aa1 and Cry48Aa1 can together act as a toxin toward the mosquito Culex quinquefasciatus and have potential use in biocontrol. Given that proteins with sequence homology to the individual proteins can have activity alone against other insect species, the structure of Tpp49Aa1 was solved in order to understand this protein more fully and inform the design of improved biopesticides. Tpp49Aa1 is naturally expressed as a crystalline inclusion within the host bacterium, and MHz serial femtosecond crystallography using the novel nanofocus option at an X-ray free electron laser allowed rapid and high-quality data collection to determine the structure of Tpp49Aa1 at 1.62 Å resolution. This revealed the packing of Tpp49Aa1 within these natural nanocrystals as a homodimer with a large intermolecular interface. Complementary experiments conducted at varied pH also enabled investigation of the early structural events leading up to the dissolution of natural Tpp49Aa1 crystals-a crucial step in its mechanism of action. To better understand the cooperation between the two proteins, assays were performed on a range of different mosquito cell lines using both individual proteins and mixtures of the two. Finally, bioassays demonstrated Tpp49Aa1/Cry48Aa1 susceptibility of Anopheles stephensi , Aedes albopictus, and Culex tarsalis larvae-substantially increasing the potential use of this binary toxin in mosquito control. Competing Interests: Competing interests statement:The authors declare no competing interest. |
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| معلومات مُعتمدة: | U01CK000516 United States ACL ACL HHS |
| فهرسة مساهمة: | Keywords: Lysinibacillus sphaericus; SFX; Tpp49Aa1; XFEL; pesticidal protein |
| المشرفين على المادة: | 0 (Pesticides) |
| SCR Organism: | Lysinibacillus sphaericus |
| تواريخ الأحداث: | Date Created: 20231128 Date Completed: 20231130 Latest Revision: 20240116 |
| رمز التحديث: | 20240117 |
| مُعرف محوري في PubMed: | PMC10710082 |
| DOI: | 10.1073/pnas.2203241120 |
| PMID: | 38015839 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.2203241120 |