دورية أكاديمية
Preventive effect of culture supernatant of epithelial-like peritoneal mesothelial cells on peritoneal fibrosis.
| العنوان: | Preventive effect of culture supernatant of epithelial-like peritoneal mesothelial cells on peritoneal fibrosis. |
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| المؤلفون: | Takahashi K; Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan., Tsuji K; Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan., Nakanoh H; Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan., Fukushima K; Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan.; Division of Nephrology, Department of Medicine, Center for Systems Biology, Program in Membrane Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA., Kitamura S; Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan.; Department of Nursing Science, Faculty of Health and Welfare Science, Okayama Prefectural University Japan., Wada J; Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan. |
| المصدر: | Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis [Perit Dial Int] 2024 May; Vol. 44 (3), pp. 211-215. Date of Electronic Publication: 2023 Nov 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: SAGE Publications Country of Publication: United States NLM ID: 8904033 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1718-4304 (Electronic) Linking ISSN: 08968608 NLM ISO Abbreviation: Perit Dial Int Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Thousand Oaks, CA] : SAGE Publications Original Publication: New York : Pergamon Press, c1988- |
| مواضيع طبية MeSH: | Peritoneal Fibrosis*/prevention & control , Peritoneal Fibrosis*/etiology , Peritoneal Fibrosis*/metabolism , Peritoneal Fibrosis*/pathology , Peritoneum*/pathology , Peritoneum*/metabolism , Epithelial Cells*/metabolism, Animals ; Mice ; Humans ; Cells, Cultured ; Mice, Nude ; Disease Models, Animal ; Male ; Peritoneal Dialysis/adverse effects |
| مستخلص: | Peritoneal fibrosis (PF) is a primary reason for discontinuing peritoneal dialysis, which involves characteristic changes of peritoneal mesothelial cells (PMCs). We previously reported preventive effects of implanting human epithelial-like PMCs (P-Epi) for mouse PF caused by mechanical peritoneum scrapings. In the present study, we analysed the preventive effects of culture supernatant of P-Epi in PF. Concentrated culture supernatant of P-Epi or human fibroblast-like PMCs (P-Fibro) or vehicles was injected into nude mice that had undergone mechanical scraping of the parietal and visceral peritoneum, and thickness and amount of adhesions were analysed. Although increased peritoneal adhesions and peritoneum thickening were observed in the vehicle-injected positive control group compared to the sham operation group, fewer number of adhesions and less thickness were observed in the mice treated with culture supernatant of P-Epi, but not P-Fibro, compared to the vehicle-injected positive controls. Immunofluorescent analysis revealed that the expression of extracellular matrix, type I collagen and fibronectin, was lower in the mice treated with culture supernatant of P-Epi than in the vehicle-injected positive controls. In addition, exosomes from P-Epi significantly reduced transforming growth factor-β (TGF-β)-induced expressions of type I collagen and fibronectin in 3T3 fibroblast cells. Collectively, culture supernatant of P-Epi has preventive effects on PF, thus cell therapy is not necessarily required. Further exploration of substances secreted by P-Epi and their protective mechanisms could lead to the development of therapeutic strategies to limit PF. Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. |
| فهرسة مساهمة: | Keywords: Peritoneal dialysis; peritoneal fibrosis; peritoneal mesothelial cells |
| تواريخ الأحداث: | Date Created: 20231129 Date Completed: 20240522 Latest Revision: 20240909 |
| رمز التحديث: | 20240910 |
| DOI: | 10.1177/08968608231213577 |
| PMID: | 38017611 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1718-4304 |
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| DOI: | 10.1177/08968608231213577 |