Environmentally Relevant Concentrations of the Insecticide Fipronil Modulated Molecular Response in Chironomus riparius.

التفاصيل البيبلوغرافية
العنوان:	Environmentally Relevant Concentrations of the Insecticide Fipronil Modulated Molecular Response in Chironomus riparius.
المؤلفون:	Pinto TJDS; PPG-SEA and NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, São Carlos, Brazil.; Analytical Chemistry Department, Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil., Martínez-Guitarte JL; Department of Physics, Mathematics, and Fluids, National Distance Education University, Madrid, Spain., Amaral Dias M; Analytical Chemistry Department, Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil., Montagner CC; Analytical Chemistry Department, Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil., Espindola ELG; PPG-SEA and NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, São Carlos, Brazil., Muñiz-González AB; Department of Physics, Mathematics, and Fluids, National Distance Education University, Madrid, Spain.; Department of Environment and Planning & CESAM, University of Aveiro, Aveiro, Portugal.
المصدر:	Environmental toxicology and chemistry [Environ Toxicol Chem] 2024 Feb; Vol. 43 (2), pp. 405-417.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: SETAC Press Country of Publication: United States NLM ID: 8308958 Publication Model: Print Cited Medium: Internet ISSN: 1552-8618 (Electronic) Linking ISSN: 07307268 NLM ISO Abbreviation: Environ Toxicol Chem Subsets: MEDLINE
أسماء مطبوعة:	Publication: Pensacola, FL : SETAC Press Original Publication: New York : Pergamon Press, c1982-
مواضيع طبية MeSH:	Insecticides/toxicity , Chironomidae/genetics , Water Pollutants, Chemical/toxicity , Pyrazoles, Animals ; Acetylcholinesterase/metabolism ; Larva/metabolism
مستخلص:	Pesticides employed worldwide for crop protection easily reach aquatic systems, which act as the main reservoirs, and become a risk factor for aquatic fauna. Fipronil is a broad-spectrum insecticide acting on the insect nervous system; however, other effects and systems unrelated to this mechanism could be affected in non-target organisms. Thus, the present study aimed to assess the impact of fipronil on the suborganismal response (gene expression and enzymatic activity) of Chironomus riparius larvae as a model organism in ecotoxicology. To this end, short-term toxicity tests were carried out with fourth-instar larvae exposed to 0.001, 0.01, and 0.1 µg L ^-1 of fipronil for 24 and 96 h. Messenger RNA levels of 42 genes related to diverse metabolic pathways were analyzed by real-time polymerase chain reaction, complemented with catalase (CAT), glutathione S-transferase (GST), and acetylcholinesterase (AChE) activities. Few effects were observed at 24 h; however, after longer exposure (96 h), genes involved in the endocrine, detoxification, stress, and immune response pathways were altered. Moreover, fipronil at 96 h increased CAT and GST activity at 0.01 µg L ^-1 and AChE at the highest concentrations. The results demonstrate that even low environmentally relevant fipronil concentrations can modulate the molecular response of several cellular pathways in C. riparius after short-term exposure. These results bring new information about the underlying response of fipronil and its mode of action on a key aquatic invertebrate. Despite no effects on mortality, strong modulation at the suborganismal level emphasizes the advantage of biomarkers as early damage responses and the harmful impact of this pesticide on freshwater organisms. Environ Toxicol Chem 2024;43:405-417. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. (© 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.)
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معلومات مُعتمدة:	PRINT/CAPES 88887.576780/2020-00 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; RTI2018-094598-B-100 Ministerio de Universidades; REGAGE21e00018296847 Universidad Nacional de Educación a Distancia
فهرسة مساهمة:	Keywords: Aquatic toxicity; Chironomids; Insecticides; Molecular response; Pesticides; Real-time polymerase chain reaction (RT-PCR)
المشرفين على المادة:	0 (Insecticides) QGH063955F (fipronil) EC 3.1.1.7 (Acetylcholinesterase) 0 (Water Pollutants, Chemical) 0 (Pyrazoles)
تواريخ الأحداث:	Date Created: 20231129 Date Completed: 20240125 Latest Revision: 20240125
رمز التحديث:	20240125
DOI:	10.1002/etc.5798
PMID:	38018734
قاعدة البيانات:	MEDLINE

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تدمد:	1552-8618
DOI:	10.1002/etc.5798