Use of alternative diluent ACP-Lact® and coconut water powder (ACP) in cryopreservation of goat semen and fixed-time artificial insemination (FTAI) in half-breed goats.

التفاصيل البيبلوغرافية
العنوان:	Use of alternative diluent ACP-Lact® and coconut water powder (ACP) in cryopreservation of goat semen and fixed-time artificial insemination (FTAI) in half-breed goats.
المؤلفون:	Cabral LAR; Faculdade de Veterinária,Universidade Estadual do Ceará, Fortaleza, CE, Brasil., Pacheco WBM; Faculdade de Veterinária,Universidade Estadual do Ceará, Fortaleza, CE, Brasil., Dos Santos SSA; Faculdade de Veterinária,Universidade Estadual do Ceará, Fortaleza, CE, Brasil., Prado ADS; Faculdade de Veterinária,Universidade Estadual do Ceará, Fortaleza, CE, Brasil., Rufino LAL; Faculdade de Veterinária,Universidade Estadual do Ceará, Fortaleza, CE, Brasil., Salgueiro CCM; Faculdade de Veterinária,Universidade Estadual do Ceará, Fortaleza, CE, Brasil., Paula NRO; Faculdade de Veterinária, Universidade Federal do Piauí, Teresina, PI, Brasil., Montenegro AR; Centro de Ciências Agrárias, Universidade Federal do Ceará, Fortaleza, CE, Brasil., Nunes JF; Faculdade de Veterinária,Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
المصدر:	Animal reproduction [Anim Reprod] 2023 Oct 20; Vol. 20 (3), pp. e20230081. Date of Electronic Publication: 2023 Oct 20 (Print Publication: 2023).
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Colégio Brasileiro de Reprodução Animal Country of Publication: Brazil NLM ID: 101272804 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-3143 (Electronic) Linking ISSN: 18069614 NLM ISO Abbreviation: Anim Reprod Subsets: PubMed not MEDLINE
أسماء مطبوعة:	Original Publication: Belo Horizonte, MG, Brazil : Colégio Brasileiro de Reprodução Animal, [2004]-
مستخلص:	The aim of the present study was to evaluate semen cryopreservation with ACP-Lact® diluent, which consists of coconut water powder (ACP) added to goat milk powder. After thawing, the samples were evaluated for sperm kinetics, membrane evaluation and in vivo insemination. For cryopreservation, a pool was made with the ejaculate of six goats, diluted in four equal aliquots for the respective treatments: T1 (ACP-Lact®); T2 (ACP-Lact® 50%); T3 (ACP + 2.5% egg yolk) and T4 (Tris + 2.5% egg yolk). After dilution of the treatments, the samples were placed in 0.5 ml straws and chilled at a rate of -1.07°C/min. After reaching 4°C and stabilizing for one hour, the straws were placed in nitrogen vapour at -60°C for 15 minutes and then immersed in liquid nitrogen (-196ºC). The straws were thawed in a 37°C water bath and kinetic assessments were performed immediately using a computerized semen analysis program (CSA), viability (EN), membrane functionality (HOST), mitochondrial activity (DAB) and DNA integrity assessment of spermatozoa. For the in vivo experiment, ten goats were inseminated, divided into two groups of five goats each, G1 inseminated with ACP-Lact® and G2 with ACP, by fixed-time artificial insemination (FTAI). Regarding the kinetic parameters, the ACP-Lact® treatment showed higher progressive motility (PM) and sperm velocity than the other treatments (36.77%). In the VSL parameter the ACP-Lact diluent was superior to ACP and Tris. In viability the treatment with ACP-Lact® was superior to the treatment with Tris, 95% and 83% respectively. In FTAI two goats were born out of the 5 goats inseminated with ACP-Lact®. It was concluded that the use of ACP-Lact® for cryopreservation of caprine semen is efficient in maintaining seminal parameters during thawing in vitro and in vivo and proved to be a good alternative extender for the caprine species. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
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فهرسة مساهمة:	Keywords: alternative diluent; cryoprotection; goat; semen freezing
تواريخ الأحداث:	Date Created: 20231129 Latest Revision: 20231201
رمز التحديث:	20231201
مُعرف محوري في PubMed:	PMC10681135
DOI:	10.1590/1984-3143-AR2023-0081
PMID:	38025996
قاعدة البيانات:	MEDLINE

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تدمد:	1984-3143
DOI:	10.1590/1984-3143-AR2023-0081