دورية أكاديمية
Using energy to go downhill-a genoprotective role for ATPase activity in DNA topoisomerase II.
العنوان: | Using energy to go downhill-a genoprotective role for ATPase activity in DNA topoisomerase II. |
---|---|
المؤلفون: | Bandak AF; Johns Hopkins University School of Medicine, Department of Biophysics and Biophysical Chemistry, Baltimore, MD 21205, USA., Blower TR; Johns Hopkins University School of Medicine, Department of Biophysics and Biophysical Chemistry, Baltimore, MD 21205, USA., Nitiss KC; Pharmaceutical Sciences Department, University of Illinois College of Pharmacy, 1601 Parkview Avenue, Rockford, IL 61107, USA.; Biomedical Sciences Department, University of Illinois College of Medicine, 1601 Parkview Avenue, Rockford, IL 61107, USA., Shah V; Pharmaceutical Sciences Department, University of Illinois College of Pharmacy, 1601 Parkview Avenue, Rockford, IL 61107, USA.; Biomedical Sciences Department, University of Illinois College of Medicine, 1601 Parkview Avenue, Rockford, IL 61107, USA., Nitiss JL; Pharmaceutical Sciences Department, University of Illinois College of Pharmacy, 1601 Parkview Avenue, Rockford, IL 61107, USA., Berger JM; Johns Hopkins University School of Medicine, Department of Biophysics and Biophysical Chemistry, Baltimore, MD 21205, USA. |
المصدر: | Nucleic acids research [Nucleic Acids Res] 2024 Feb 09; Vol. 52 (3), pp. 1313-1324. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 1992- : Oxford : Oxford University Press Original Publication: London, Information Retrieval ltd. |
مواضيع طبية MeSH: | DNA*/genetics , DNA Topoisomerases, Type II*/genetics, Humans ; Adenosine Triphosphatases/genetics ; Adenosine Triphosphate ; Etoposide/pharmacology ; DNA Damage |
مستخلص: | Type II topoisomerases effect topological changes in DNA by cutting a single duplex, passing a second duplex through the break, and resealing the broken strand in an ATP-coupled reaction cycle. Curiously, most type II topoisomerases (topos II, IV and VI) catalyze DNA transformations that are energetically favorable, such as the removal of superhelical strain; why ATP is required for such reactions is unknown. Here, using human topoisomerase IIβ (hTOP2β) as a model, we show that the ATPase domains of the enzyme are not required for DNA strand passage, but that their loss elevates the enzyme's propensity for DNA damage. The unstructured C-terminal domains (CTDs) of hTOP2β strongly potentiate strand passage activity in ATPase-less enzymes, as do cleavage-prone mutations that confer hypersensitivity to the chemotherapeutic agent etoposide. The presence of either the CTD or the mutations lead ATPase-less enzymes to promote even greater levels of DNA cleavage in vitro, as well as in vivo. By contrast, aberrant cleavage phenotypes of these topo II variants is significantly repressed when the ATPase domains are present. Our findings are consistent with the proposal that type II topoisomerases acquired ATPase function to maintain high levels of catalytic activity while minimizing inappropriate DNA damage. (© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.) |
التعليقات: | Update of: bioRxiv. 2023 Jun 27;:. (PMID: 37425896) Erratum in: Nucleic Acids Res. 2023 Dec 28;:. (PMID: 38153185) |
References: | Mol Cell Biol. 2015 Aug;35(16):2799-817. (PMID: 26055322) Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4057-62. (PMID: 8633016) Exp Cell Res. 1999 Sep 15;251(2):329-39. (PMID: 10471318) Nucleic Acids Res. 1990 Oct 25;18(20):6069-74. (PMID: 2235490) Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14416-21. (PMID: 8962066) Gene. 1992 Jan 2;110(1):119-22. (PMID: 1544568) Mol Gen Genet. 1996 Aug 27;252(1-2):79-86. (PMID: 8804406) Nature. 1996 Jan 18;379(6562):225-32. (PMID: 8538787) Nucleic Acids Res. 2009 Feb;37(3):679-92. (PMID: 19208647) Anal Chem. 2017 Sep 19;89(18):9976-9983. (PMID: 28803470) Cell. 1990 Aug 10;62(3):403-6. (PMID: 2165864) Bioessays. 1998 Mar;20(3):215-26. (PMID: 9631649) J Biol Chem. 2005 Nov 25;280(47):39337-45. (PMID: 16188892) J Biomol Screen. 2006 Mar;11(2):131-7. (PMID: 16490773) Biophys Chem. 2007 Dec;131(1-3):62-70. (PMID: 17904724) Biochim Biophys Acta. 2012 Jul;1819(7):632-8. (PMID: 22233557) Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2302064120. (PMID: 37406101) J Biol Chem. 1982 May 25;257(10):5866-72. (PMID: 6279616) Annu Rev Biochem. 2013;82:139-70. (PMID: 23495937) Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71. (PMID: 200930) Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6. (PMID: 186775) J Biol Chem. 1993 Nov 15;268(32):24481-90. (PMID: 8227000) Biochem Soc Trans. 2010 Apr;38(2):438-42. (PMID: 20298198) Crit Rev Biochem Mol Biol. 1991;26(3-4):335-75. (PMID: 1657531) Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6. (PMID: 337300) Nat Commun. 2021 May 20;12(1):2962. (PMID: 34016969) Proc Natl Acad Sci U S A. 2004 May 11;101(19):7293-8. (PMID: 15123801) Nucleic Acids Res. 2011 Aug;39(15):6327-39. (PMID: 21525132) Nature. 1997 Aug 28;388(6645):903-6. (PMID: 9278055) Proc Natl Acad Sci U S A. 1979 Dec;76(12):6110-4. (PMID: 230498) Adv Pharmacol. 1994;29B:257-70. (PMID: 8996612) Elife. 2022 Nov 07;11:. (PMID: 36342377) Cell. 1980 Aug;21(1):103-14. (PMID: 6250706) Proc Natl Acad Sci U S A. 2020 Oct 27;117(43):26876-26884. (PMID: 33046655) Proc Natl Acad Sci U S A. 1987 Oct;84(20):7024-7. (PMID: 2823250) Biochimie. 2007 Apr;89(4):427-46. (PMID: 17293019) J Biol Chem. 2004 Jun 11;279(24):25783-8. (PMID: 15047688) Science. 2006 Jun 23;312(5781):1798-802. (PMID: 16794079) Bioessays. 1991 Jun;13(6):269-73. (PMID: 1654050) Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834) J Biol Chem. 1980 Jun 10;255(11):4976-9. (PMID: 6246090) Cell. 1980 Mar;19(3):697-707. (PMID: 6244895) Nature. 1991 Jun 20;351(6328):624-9. (PMID: 1646964) Nat Methods. 2009 May;6(5):343-5. (PMID: 19363495) Cancer Chemother Pharmacol. 1997;39(4):367-75. (PMID: 9025779) Proc Natl Acad Sci U S A. 1981 Feb;78(2):843-7. (PMID: 6262776) Nat Rev Mol Cell Biol. 2016 Nov;17(11):703-721. (PMID: 27649880) Cell. 1994 May 20;77(4):609-16. (PMID: 8187179) Nucleic Acids Res. 2007;35(11):3810-22. (PMID: 17526531) Nucleic Acids Res. 2013 Sep;41(16):7815-27. (PMID: 23804759) Nat Genet. 2010 Aug;42(8):668-75. (PMID: 20601956) |
معلومات مُعتمدة: | R01 CA077373 United States CA NCI NIH HHS; R03 CA259884 United States CA NCI NIH HHS; R03 NS116666 United States NS NINDS NIH HHS; T32-GM008403-28 United States NH NIH HHS |
المشرفين على المادة: | EC 3.6.1.- (Adenosine Triphosphatases) 8L70Q75FXE (Adenosine Triphosphate) 9007-49-2 (DNA) EC 5.99.1.3 (DNA Topoisomerases, Type II) 6PLQ3CP4P3 (Etoposide) |
تواريخ الأحداث: | Date Created: 20231201 Date Completed: 20240214 Latest Revision: 20240219 |
رمز التحديث: | 20240219 |
مُعرف محوري في PubMed: | PMC10853770 |
DOI: | 10.1093/nar/gkad1157 |
PMID: | 38038260 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1362-4962 |
---|---|
DOI: | 10.1093/nar/gkad1157 |