دورية أكاديمية
Comparative analysis of Chrysoporthe cubensis exoproteomes and their specificity for saccharification of sugarcane bagasse.
العنوان: | Comparative analysis of Chrysoporthe cubensis exoproteomes and their specificity for saccharification of sugarcane bagasse. |
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المؤلفون: | Tavares MP; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil., Morgan T; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil., Gomes RF; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil., Mendes JPR; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil., Castro-Borges W; Department of Biological Science, Universidade Federal de Ouro Preto, Campus Universitário Morro do Cruzeiro, 35400-000 Ouro Preto, MG, Brasil., Maitan-Alfenas GP; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil., Guimarães VM; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36570-900 Viçosa, MG, Brazil. Electronic address: vmonteze@ufv.br. |
المصدر: | Enzyme and microbial technology [Enzyme Microb Technol] 2024 Feb; Vol. 173, pp. 110365. Date of Electronic Publication: 2023 Nov 22. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8003761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0909 (Electronic) Linking ISSN: 01410229 NLM ISO Abbreviation: Enzyme Microb Technol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: New York, NY : Elsevier Original Publication: [Guildford, Eng.] IPC Science and Technology Press. |
مواضيع طبية MeSH: | Cellulose*/metabolism , Saccharum*/metabolism, Proteomics ; Tandem Mass Spectrometry ; Fungal Proteins/metabolism ; Dietary Fiber/metabolism ; Hydrolysis |
مستخلص: | The phytopathogenic fungus Chrysoporthe cubensis is a relevant source of lignocellulolytic enzymes. This work aimed to compare the profile of lignocellulose-degrading proteins secreted by C. cubensis grown under semi-solid state fermentation using wheat bran (WB) and sugarcane bagasse (SB). The exoproteomes of the fungus grown in wheat bran (WBE) and sugarcane bagasse (SBE) were qualitative and quantitatively analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Data are available via ProteomeXchange with identifier PXD046075. Label-free proteomic analysis of WBE and SBE showed that the fungus produced a spectrum of carbohydrate-active enzymes (CAZymes) with exclusive characteristics from each extract. While SBE resulted in an enzymatic profile directed towards the depolymerization of cellulose, the enzymes in WBE were more adaptable to the degradation of biomass rich in hemicellulose and other non-lignocellulosic polymers. Saccharification of alkaline pre-treated sugarcane bagasse with SBE promoted glucose release higher than commercial cocktails (8.11 g L -1 ), while WBE promoted the higher release of xylose (5.71 g L -1 ). Our results allowed an in-depth knowledge of the complex set of enzymes secreted by C. cubensis responsible for its high lignocellulolytic activity and still provided the identification of promising target proteins for biotechnological applications in the context of biorefinery. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
فهرسة مساهمة: | Keywords: Biomass saccharification; CAZymes; Chrysoporthe cubensis; Exoproteome; Sugarcane bagasse; Wheat bran |
المشرفين على المادة: | 9006-97-7 (bagasse) 9004-34-6 (Cellulose) 0 (Fungal Proteins) 0 (Dietary Fiber) |
SCR Organism: | Chrysoporthe cubensis |
تواريخ الأحداث: | Date Created: 20231203 Date Completed: 20231225 Latest Revision: 20231225 |
رمز التحديث: | 20231225 |
DOI: | 10.1016/j.enzmictec.2023.110365 |
PMID: | 38043248 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1879-0909 |
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DOI: | 10.1016/j.enzmictec.2023.110365 |