دورية أكاديمية
Changes of miR-139-5p, TGFB1, and COL1A1 in the placental tissue of cases with gestational diabetes mellitus.
| العنوان: | Changes of miR-139-5p, TGFB1, and COL1A1 in the placental tissue of cases with gestational diabetes mellitus. |
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| المؤلفون: | Akin MN; Mugla Sitki Kocman University, Faculty of Medicine, Department of Gynaecology and Obstetrics, Mugla, Turkey., Kasap B; Mugla Sitki Kocman University, Faculty of Medicine, Department of Gynaecology and Obstetrics, Mugla, Turkey., Pirincci F; Mugla Sitki Kocman University, Faculty of Medicine, Department of Gynaecology and Obstetrics, Mugla, Turkey., Sezgin B; Mugla Sitki Kocman University, Faculty of Medicine, Department of Gynaecology and Obstetrics, Mugla, Turkey., Ozdemir C; Muğla Sıtkı Koçman University, Health Sciences Institution, Department of Medical Biology, Mugla, Turkey., Demirtas Bilgic A; Muğla Sıtkı Koçman University, Health Sciences Institution, Department of Medical Biology, Mugla, Turkey., Aftabi Y; Tabriz University of Medical Sciences, Tuberculosis and Lung Diseases Research Center, Tabriz, Iran., Gokdogan Edgunlu T; Mugla Sitki Kocman University, Faculty of Medicine, Department of Medical Biology, Mugla, Turkey. Electronic address: tedgunlu@gmail.com. |
| المصدر: | Gene [Gene] 2024 Mar 01; Vol. 897, pp. 148061. Date of Electronic Publication: 2023 Dec 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Diabetes, Gestational*/genetics , MicroRNAs*/genetics , MicroRNAs*/metabolism , Transforming Growth Factor beta1*/genetics , Collagen Type I, alpha 1 Chain*/genetics, Female ; Humans ; Pregnancy ; Placenta/metabolism ; RNA, Messenger |
| مستخلص: | There are structural changes in the placenta of cases with Gestational Diabetes Mellitus (GDM). TGF-β and collagen pathways have crucial roles in tissue remodeling and TGF-β1 and COL1A1 are important genes in these signalling respectively. Also, lncRNA NEAT1, and miRNA hsa-miR-139-5p and hsa-miR-129-5p have regulatory effects on TGF-β1 and COL1A1. Here we aimed to assess their expressions in the placenta tissue of GDM cases. 30 patients with GDM and 30 healthy pregnant women participated in the study. Placental tissues taken during normal or cesarean delivery were used and total RNA was isolated from the tissues. mRNA levels were determined by qPCR and protein levels were determined by ELISA methods. An in silico analysis was done to elucidate the possible relation of TGF-β1 and COL1A1 gene networks with GDM. We determined that NEAT1 and miR-129-5p expression levels did not differ between GDM and healthy control groups (p = 0.697 and 0.412, respectively). But, miR-139-5p mRNA level, TGFB1 and COL1A1 protein levels significantly differ between the GDM and control groups (p = 0.000, p = 0.000 and p = 0.001, respectively). The in silico analysis revealed that TGFB1 and COL1A1 genes network may have important role in the GDM with their variety of members and regulatory molecules NEAT1, hsa-miR-139-5p, and hsa-miR-129-5p can control their functions. The expression of TGFB1, COL1A1 and miR-139-5p is changed in placenta tissue of GDM cases and many genes in the interacting networks of TGFB1 and COL1A1 could contribute to the pathogenicity of GDM. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: COL1A1; Gestational diabetes mellitus; In silico; TGF-β |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (Mirn129 microRNA, human) 0 (MIRN139 microRNA, human) 0 (RNA, Messenger) 0 (TGFB1 protein, human) 0 (Transforming Growth Factor beta1) 0 (COL1A1 protein, human) 0 (Collagen Type I, alpha 1 Chain) |
| تواريخ الأحداث: | Date Created: 20231204 Date Completed: 20240129 Latest Revision: 20240129 |
| رمز التحديث: | 20240129 |
| DOI: | 10.1016/j.gene.2023.148061 |
| PMID: | 38048967 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0038 |
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| DOI: | 10.1016/j.gene.2023.148061 |