دورية أكاديمية
Combining experiment and energy landscapes to explore anaerobic heme breakdown in multifunctional hemoproteins.
| العنوان: | Combining experiment and energy landscapes to explore anaerobic heme breakdown in multifunctional hemoproteins. |
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| المؤلفون: | Keith AD; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk., Sawyer EB; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk., Choy DCY; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk., Xie Y; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk., Biggs GS; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk., Klein OJ; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk., Brear PD; Department of Biochemistry, University of Cambridge, Sanger Building, Cambridge CB2 1GA, UK., Wales DJ; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk., Barker PD; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK. adk44@cam.ac.uk. |
| المصدر: | Physical chemistry chemical physics : PCCP [Phys Chem Chem Phys] 2024 Jan 03; Vol. 26 (2), pp. 695-712. Date of Electronic Publication: 2024 Jan 03. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 100888160 Publication Model: Electronic Cited Medium: Internet ISSN: 1463-9084 (Electronic) Linking ISSN: 14639076 NLM ISO Abbreviation: Phys Chem Chem Phys Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge [England] : Royal Society of Chemistry, c1999- |
| مواضيع طبية MeSH: | Hemeproteins*/metabolism, Anaerobiosis ; Phylogeny ; Heme/metabolism ; Escherichia coli/metabolism |
| مستخلص: | To survive, many pathogens extract heme from their host organism and break down the porphyrin scaffold to sequester the Fe 2+ ion via a heme oxygenase. Recent studies have revealed that certain pathogens can anaerobically degrade heme. Our own research has shown that one such pathway proceeds via NADH-dependent heme degradation, which has been identified in a family of hemoproteins from a range of bacteria. HemS, from Yersinia enterocolitica , is the main focus of this work, along with HmuS ( Yersinia pestis ), ChuS ( Escherichia coli ) and ShuS ( Shigella dysenteriae ). We combine experiments, Energy Landscape Theory, and a bioinformatic investigation to place these homologues within a wider phylogenetic context. A subset of these hemoproteins are known to bind certain DNA promoter regions, suggesting not only that they can catalytically degrade heme, but that they are also involved in transcriptional modulation responding to heme flux. Many of the bacterial species responsible for these hemoproteins (including those that produce HemS, ChuS and ShuS) are known to specifically target oxygen-depleted regions of the gastrointestinal tract. A deeper understanding of anaerobic heme breakdown processes exploited by these pathogens could therefore prove useful in the development of future strategies for disease prevention. |
| المشرفين على المادة: | 0 (Hemeproteins) 42VZT0U6YR (Heme) |
| تواريخ الأحداث: | Date Created: 20231206 Date Completed: 20240104 Latest Revision: 20240104 |
| رمز التحديث: | 20240104 |
| DOI: | 10.1039/d3cp03897a |
| PMID: | 38053511 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1463-9084 |
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| DOI: | 10.1039/d3cp03897a |