دورية أكاديمية
A ToxIN homolog from Salmonella enterica serotype Enteritidis impairs bacteriophage infection.
| العنوان: | A ToxIN homolog from Salmonella enterica serotype Enteritidis impairs bacteriophage infection. |
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| المؤلفون: | McFarlane JA; BioTechnology Institute, University of Minnesota, Saint Paul, MN 55108, USA., Hansen EG; Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN 55108, USA., Ortega EC; Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN 55108, USA., Iskender I; Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN 55108, USA., Noireaux V; School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA., Bowden SD; BioTechnology Institute, University of Minnesota, Saint Paul, MN 55108, USA.; Department of Food Science and Nutrition, University of Minnesota, Saint Paul, MN 55108, USA. |
| المصدر: | Journal of applied microbiology [J Appl Microbiol] 2023 Dec 01; Vol. 134 (12). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: In Process; MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| مستخلص: | Aims: To determine if the bacteriophage abortive infection system ToxIN is present in foodborne Salmonella and if it protects against infection by bacteriophages specific to enteric bacteria. Methods and Results: A set of foodborne Salmonella enteritidis isolates from a 2010 eggshell outbreak was identified via BLASTN (basic local alignment search tool nucleotide) queries as harboring a close homolog of ToxIN, carried on a plasmid with putative mobilization proteins. This homolog was cloned into a plasmid vector and transformed into the laboratory strain Salmonella typhimurium LT2 and tested against a set of Salmonella-specific phages (FelixO1, S16, Sp6, LPST153, and P22 HT105/1 int-201). ToxIN reduced infection by FelixO1, S16, and LPST153 by ∼1-4 log PFU ml-1 while reducing the plaque size of Sp6. When present in LT2 and Escherichia coli MG1655, ToxIN conferred cross-genus protection against phage isolates, which infect both bacteria. Finally, the putative ToxIN plasmid was found in whole-genome sequence contigs of several Salmonella serovars, pathogenic E. coli, and other pathogenic enterobacteria. Conclusions: Salmonella and E. coli can resist infection by several phages via ToxIN under laboratory conditions; ToxIN is present in foodborne pathogens including Salmonella and Shiga-toxigenic E. coli. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.) |
| معلومات مُعتمدة: | Minnesota's Discovery, Research, and InnoVation Economy; BioTechnology Institute, University of Minnesota; Dr. Elwood F. Caldwell Graduate Fellowship and the Ted Labuza Assistantship; Department of Food Science and Nutrition, University of Minnesota; 2228971 National Science Foundation; Department of Food Science and Nutrition and the College of Food, Agriculture, and Natural Resource Sciences, University of Minnesota |
| فهرسة مساهمة: | Keywords: Salmonella; ToxIN; abortive infection; bacteriophage; foodborne; phage resistance |
| تواريخ الأحداث: | Date Created: 20231207 Latest Revision: 20231216 |
| رمز التحديث: | 20231216 |
| DOI: | 10.1093/jambio/lxad299 |
| PMID: | 38059866 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1365-2672 |
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| DOI: | 10.1093/jambio/lxad299 |