دورية أكاديمية
[The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin].
العنوان: | [The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin]. |
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المؤلفون: | Novoselova EG; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia.; elenanov_06@mail.ru., Glushkova OV; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia., Khrenov MO; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia., Lunin SM; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia., Sharapov MG; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia., Goncharov RG; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia., Mubarakshina EK; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia., Novoselova TV; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia., Parfenyuk SB; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia. |
المصدر: | Molekuliarnaia biologiia [Mol Biol (Mosk)] 2023 Nov-Dec; Vol. 57 (6), pp. 1006-1016. |
نوع المنشور: | English Abstract; Journal Article |
اللغة: | Russian |
بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0105454 Publication Model: Print Cited Medium: Print ISSN: 0026-8984 (Print) Linking ISSN: 00268984 NLM ISO Abbreviation: Mol Biol (Mosk) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
مواضيع طبية MeSH: | NF-kappa B*/genetics , NF-kappa B*/metabolism , Thymosin*/genetics , Thymosin*/pharmacology, Animals ; Mice ; RAW 264.7 Cells ; Endotoxins ; Lipopolysaccharides/pharmacology ; Cytokines/metabolism |
مستخلص: | The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the Tlr4 gene. Third, Tα1 significantly reduced p53 and the activity of the P53 gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in Ar-1 gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells. |
فهرسة مساهمة: | Keywords: cytokines; gene expression; inflammation; signaling pathways; thymosin-1α |
المشرفين على المادة: | 0 (NF-kappa B) 0 (Endotoxins) 0 (Lipopolysaccharides) 61512-21-8 (Thymosin) 0 (Cytokines) |
تواريخ الأحداث: | Date Created: 20231208 Date Completed: 20231216 Latest Revision: 20231216 |
رمز التحديث: | 20231217 |
DOI: | 10.31857/S0026898423060125, EDN: QGCMUF |
PMID: | 38062956 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0026-8984 |
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DOI: | 10.31857/S0026898423060125, EDN: QGCMUF |