دورية أكاديمية
Follicular fluid C3a-peptide promotes oocyte maturation through F-actin aggregation.
| العنوان: | Follicular fluid C3a-peptide promotes oocyte maturation through F-actin aggregation. |
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| المؤلفون: | Yang Y; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China.; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China., Zhao C; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China., Chen B; Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Anhui, China., Yu X; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China.; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China., Zhou Y; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China., Ni D; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China., Zhang X; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China., Zhang J; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China., Ling X; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Reproduction, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu Province, 210004, China. lingxiufeng_njfy@163.com., Zhang Z; Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Anhui, China. 18155106683@163.com., Huo R; State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn.; Suzhou Affiliated Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School,, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn. |
| المصدر: | BMC biology [BMC Biol] 2023 Dec 08; Vol. 21 (1), pp. 285. Date of Electronic Publication: 2023 Dec 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101190720 Publication Model: Electronic Cited Medium: Internet ISSN: 1741-7007 (Electronic) Linking ISSN: 17417007 NLM ISO Abbreviation: BMC Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : BioMed Central, c2003- |
| مواضيع طبية MeSH: | Actins*/metabolism , Follicular Fluid*/physiology , Oocytes*/growth & development , Complement C3a*/physiology , Peptide Fragments*/physiology, Animals ; Female ; Humans ; Mice ; Chromatography, Liquid ; Cumulus Cells/metabolism ; Tandem Mass Spectrometry ; In Vitro Oocyte Maturation Techniques |
| مستخلص: | Background: Immature cumulus-oocyte complexes are retrieved to obtain mature oocytes by in vitro maturation (IVM), a laboratory tool in reproductive medicine to obtain mature oocytes. Unfortunately, the efficiency of IVM is not satisfactory. To circumvent this problem, we therefore intended to commence with the composition of ovarian follicular fluid (FF), an important microenvironment influencing oocyte growth. It is well known that FF has a critical role in oocyte development and maturation. However, the components in human FF remain largely unknown, particularly with regard to small molecular peptides. Results: In current study, the follicular fluid derived from human mature and immature follicles were harvested. The peptide profiles of FF were further investigated by using combined ultrafiltration and LC-MS/MS. The differential peptides were preliminary determined by performing differentially expressed analysis. Human and mouse oocyte culture were used to verify the influence of differential peptides on oocyte development. Constructing plasmids, cell transfecting, Co-IP, PLA etc. were used to reveal the detail molecular mechanism. The results from differentially expressed peptide as well as cultured human and mouse oocytes analyses showed that highly conserved C3a-peptide, a cleavage product of complement C3a, definitely affected oocytes development. Intriguingly, C3a-peptide possessed a novel function that promoted F-actin aggregation and spindle migration, raised the percentage of oocytes at the MII stage, without increasing the chromosome aneuploidy ratio, especially in poor-quality oocytes. These effects of C3a-peptide were attenuated by C3aR morpholino inhibition, suggesting that C3a-peptide affected oocytes development by collaborating with its classical receptor, C3aR. Specially, we found that C3aR co-localized to the spindle with β-tubulin to recruit F-actin toward the spindle and subcortical region of the oocytes through specific binding to MYO10, a key regulator for actin organization, spindle morphogenesis and positioning in oocytes. Conclusions: Our results provide a new perspective for improving IVM culture systems by applying FF components and also provide molecular insights into the physiological function of C3a-peptide, its interaction with C3aR, and their roles in enabling meiotic division of oocytes. (© 2023. The Author(s).) |
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| فهرسة مساهمة: | Keywords: C3a-peptide; C3aR; Human follicular fluid; Liquid chromatography-tandem mass spectrometry; MYO10; Oocyte in vitro maturation |
| المشرفين على المادة: | 0 (Actins) 80295-42-7 (Complement C3a) 0 (Peptide Fragments) |
| تواريخ الأحداث: | Date Created: 20231209 Date Completed: 20240112 Latest Revision: 20240112 |
| رمز التحديث: | 20240113 |
| مُعرف محوري في PubMed: | PMC10709936 |
| DOI: | 10.1186/s12915-023-01760-6 |
| PMID: | 38066646 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1741-7007 |
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| DOI: | 10.1186/s12915-023-01760-6 |