دورية أكاديمية
"Antibacterial effect and possible mechanism of action of 1,3,4-oxadiazole in Staphylococcus aureus".
| العنوان: | "Antibacterial effect and possible mechanism of action of 1,3,4-oxadiazole in Staphylococcus aureus". |
|---|---|
| المؤلفون: | Formagio MD; Postgraduate Program of Health Science, State University of Maringá, Maringá, Paraná, Brazil, 87020-900., Silva JVO; Postgraduate Program of Health Science, State University of Maringá, Maringá, Paraná, Brazil, 87020-900., Silva AF; Department of Agricultural and Natural Sciences, State University of Minas Gerais, Ituiutaba, Minas Gerais, Brazil, 38302-192., Campanerut-Sá PAZ; Postgraduate Program of Health Science, State University of Maringá, Maringá, Paraná, Brazil, 87020-900.; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá Paraná, Brazil, 87020-900., Urbano A; Physics Department, State University of Londrina, Londrina, Brazil, 86057-970., Bonfim-Mendonça PS; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá Paraná, Brazil, 87020-900., Capoci IRG; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá Paraná, Brazil, 87020-900., Cotica ÉSK; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá Paraná, Brazil, 87020-900., Mikcha JMG; Postgraduate Program of Health Science, State University of Maringá, Maringá, Paraná, Brazil, 87020-900.; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá Paraná, Brazil, 87020-900. |
| المصدر: | Letters in applied microbiology [Lett Appl Microbiol] 2024 Jan 02; Vol. 77 (1). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8510094 Publication Model: Print Cited Medium: Internet ISSN: 1472-765X (Electronic) Linking ISSN: 02668254 NLM ISO Abbreviation: Lett Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Oxford : Oxford University Press Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985- |
| مواضيع طبية MeSH: | Staphylococcus aureus* , Staphylococcal Infections*, Humans ; Reactive Oxygen Species ; Anti-Bacterial Agents/pharmacology ; Oxadiazoles/pharmacology ; Microbial Sensitivity Tests |
| مستخلص: | Staphylococcus aureus is one of the main etiological agents causing foodborne diseases, and the development of new antibacterial agents is urgent. This study evaluated the antibacterial activity and the possible mechanism of action of the 1,3,4-oxadiazole LMM6 against S. aureus. The minimum inhibitory concentration (MIC) of LMM6 ranged from 1.95 to 7.81 µg ml-1. The time-kill assay showed that 48-h treatment at 1× to 8× MIC reduced S. aureus by 4 log colony forming unit (CFU), indicating a bacteriostatic effect. Regarding the possible mechanism of action of LMM6, there was accumulation of reactive oxygen species (ROS) and an increase in the absorption of crystal violet (∼50%) by the cells treated with LMM6 at 1× and 2× MIC for 6-12 h. In addition, there was increased propidium iodide uptake (∼84%) after exposure to LMM6 for 12 h at 2× MIC. After 48 h of treatment, 100% of bacteria had been injured. Scanning electron microscopy observations demonstrated that LMM6-treated cells were smaller compared with the untreated group. LMM6 exhibited bacteriostatic activity and its mechanism of action involves increase of intracellular ROS and disturbance of the cell membrane, which can be considered a key target for controlling the growth of S. aureus. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.) |
| معلومات مُعتمدة: | 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| فهرسة مساهمة: | Keywords: S. aureus; 1,3,4-oxadiazole; LMM6; antibacterial activity; mechanism of action |
| المشرفين على المادة: | 20O2F20OUR (1,3,4-oxadiazole) 0 (Reactive Oxygen Species) 0 (Anti-Bacterial Agents) 0 (Oxadiazoles) |
| تواريخ الأحداث: | Date Created: 20231209 Date Completed: 20240103 Latest Revision: 20240103 |
| رمز التحديث: | 20240103 |
| DOI: | 10.1093/lambio/ovad138 |
| PMID: | 38070878 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1472-765X |
|---|---|
| DOI: | 10.1093/lambio/ovad138 |