دورية أكاديمية
Coordinated upregulation of two CDP-diacylglycerol synthases, YnbB and CdsA, is essential for cell growth and membrane protein export in the cold.
| العنوان: | Coordinated upregulation of two CDP-diacylglycerol synthases, YnbB and CdsA, is essential for cell growth and membrane protein export in the cold. |
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| المؤلفون: | Kamemoto Y; The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020-8550, Japan., Hikage R; Department of Applied Biological Chemistry and Food Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan., Han Y; Department of Applied Biological Chemistry and Food Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan., Sekiya Y; Department of Applied Biological Chemistry and Food Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan., Sawasato K; Department of Applied Biological Chemistry and Food Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan., Nishiyama KI; The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020-8550, Japan.; Department of Applied Biological Chemistry and Food Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan. |
| المصدر: | FEMS microbiology letters [FEMS Microbiol Lett] 2023 Jan 17; Vol. 370. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7705721 Publication Model: Print Cited Medium: Internet ISSN: 1574-6968 (Electronic) Linking ISSN: 03781097 NLM ISO Abbreviation: FEMS Microbiol Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Oxford Oxford University Press Original Publication: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies. |
| مواضيع طبية MeSH: | Membrane Proteins*/genetics , Membrane Proteins*/metabolism , Diacylglycerol Cholinephosphotransferase*/genetics , Diacylglycerol Cholinephosphotransferase*/metabolism, Cytidine Diphosphate Diglycerides ; Up-Regulation ; Glycolipids/metabolism |
| مستخلص: | YnbB is a paralogue of CdsA, a CDP-diacylglycerol synthase. While the cdsA gene is essential, the ynbB gene is dispensable. So far, no phenotype of ynbB knockout has been observed. We found that a ynbB knockout strain acquired cold-sensitivity on growth under CdsA-limited conditions. We found that MPIase, a glycolipid involved in protein export, is cold-upregulated to facilitate protein export in the cold, by increasing the mRNA levels of not only CdsA but also that of YnbB. Under non-permissive conditions, phospholipid biosynthesis proceeded normally, however, MPIase upregulation was inhibited with accumulation of precursors of membrane and secretory proteins such as M13 procoat and proOmpA, indicating that YnbB is dedicated to MPIase biosynthesis, complementing the CdsA function. (© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.) |
| معلومات مُعتمدة: | 22H02567 JSPS |
| فهرسة مساهمة: | Keywords: CDP-diacylglycerol synthase; CdsA; MPIase; YnbB; phospholipids; protein insertion and translocation |
| المشرفين على المادة: | 0 (Membrane Proteins) EC 2.7.8.2 (Diacylglycerol Cholinephosphotransferase) 0 (Cytidine Diphosphate Diglycerides) 0 (Glycolipids) |
| تواريخ الأحداث: | Date Created: 20231209 Date Completed: 20231229 Latest Revision: 20240208 |
| رمز التحديث: | 20240208 |
| DOI: | 10.1093/femsle/fnad131 |
| PMID: | 38070879 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1574-6968 |
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| DOI: | 10.1093/femsle/fnad131 |