دورية أكاديمية
Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.
| العنوان: | Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding. |
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| المؤلفون: | Wang P; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China; School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, China., Fan N; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China., Yang W; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China., Cao P; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China., Liu G; School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, China., Zhao Q; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China; State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China., Guo P; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China; State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China., Li X; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China; Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animals, Hohhot, China., Lin X; State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China., Jiang N; State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China. Electronic address: ningjiang@fudan.edu.cn., Nashun B; Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China. Electronic address: bnashun@imu.edu.cn. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2024 Jan; Vol. 300 (1), pp. 105538. Date of Electronic Publication: 2023 Dec 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | CCCTC-Binding Factor*/genetics , CCCTC-Binding Factor*/metabolism , Chromatin*/genetics , DNA-Binding Proteins*/genetics , Gene Expression Regulation* , High Mobility Group Proteins*/genetics , Histone Chaperones*/genetics, Animals ; Mice ; DNA Repair ; DNA Replication ; NIH 3T3 Cells |
| مستخلص: | Histone chaperone FACT (facilitates chromatin transcription) is well known to promote chromatin recovery during transcription. However, the mechanism how FACT regulates genome-wide chromatin accessibility and transcription factor binding has not been fully elucidated. Through loss-of-function studies, we show here that FACT component Ssrp1 is required for DNA replication and DNA damage repair and is also essential for progression of cell phase transition and cell proliferation in mouse embryonic fibroblast cells. On the molecular level, absence of the Ssrp1 leads to increased chromatin accessibility, enhanced CTCF binding, and a remarkable change in dynamic range of gene expression. Our study thus unequivocally uncovers a unique mechanism by which FACT complex regulates transcription by coordinating genome-wide chromatin accessibility and CTCF binding. Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CTCF; FACT; Ssrp1; chromatin accessibility; histone chaperone; transcription |
| المشرفين على المادة: | 0 (CCCTC-Binding Factor) 0 (Chromatin) 0 (DNA-Binding Proteins) 0 (High Mobility Group Proteins) 0 (Histone Chaperones) 143107-87-3 (Ssrp1 protein, mouse) 0 (Ctcf protein, mouse) |
| تواريخ الأحداث: | Date Created: 20231210 Date Completed: 20240209 Latest Revision: 20240701 |
| رمز التحديث: | 20240701 |
| مُعرف محوري في PubMed: | PMC10808957 |
| DOI: | 10.1016/j.jbc.2023.105538 |
| PMID: | 38072046 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1083-351X |
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| DOI: | 10.1016/j.jbc.2023.105538 |