دورية أكاديمية
Expression of mitochondrial transcription factor A in granulosa cells: implications for oocyte maturation and in vitro fertilization outcomes.
| العنوان: | Expression of mitochondrial transcription factor A in granulosa cells: implications for oocyte maturation and in vitro fertilization outcomes. |
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| المؤلفون: | Wang CM; Department of Reproductive Medicine, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, 050011, China., Liu CM; Department of Obstetrics, The Fourth Hospital of Shijiazhuang, Shijiazhuang, 050011, China., Jia XZ; Department of Reproductive Medicine, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, 050011, China., Zhao SB; Department of Reproductive Medicine, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, 050011, China., Nie ZY; Department of Reproductive Medicine, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, 050011, China., Lv CT; Department of Reproductive Medicine, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, 050011, China., Jiang Q; Department of Reproductive Medicine, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, 050011, China., Hao YL; Department of Reproductive Medicine, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, 050011, China. haoyali@hebmu.edu.cn. |
| المصدر: | Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2024 Feb; Vol. 41 (2), pp. 363-370. Date of Electronic Publication: 2023 Dec 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 9206495 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-7330 (Electronic) Linking ISSN: 10580468 NLM ISO Abbreviation: J Assist Reprod Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004- >: Amsterdam : Springer Original Publication: New York : Plenum Press, c1992- |
| مواضيع طبية MeSH: | Fertilization in Vitro* , Infertility*/therapy , DNA-Binding Proteins* , Transcription Factors* , Mitochondrial Proteins*, Pregnancy ; Humans ; Female ; Granulosa Cells/metabolism ; Oocytes/metabolism ; RNA, Messenger/metabolism |
| مستخلص: | Objective: In vitro fertilization-embryo transfer (IVF-ET) is a widely used treatment for infertility, with oocyte maturation and quality having a significant impact on oocyte fertilization, embryo development, and fetal growth. Mitochondrial transcription factor A (TFAM) is essential for maintaining the mitochondrial oxidative respiratory chain and supplying energy for oocyte development, fertilization, and embryonic development. In this study, we aimed to examine TFAM expression in women undergoing IVF-ET and assess its impact on the IVF outcomes. Methods: We recruited 85 women who underwent IVF-ET treatment for infertility. On the date of egg collection, granulosa cells were extracted from the clear follicular fluid of the first mature egg using ultrasound-guided needle aspiration. The collected granulosa cells served three purposes: (1) detecting TFAM gene expression in granulosa cells via immunocytochemistry, (2) determining TFAM mRNA expression using reverse transcription-PCR (RT-PCR), and (3) measuring TFAM protein expression through western blotting. Result: Based on the results, we found that TFAM was localized and expressed in the cytoplasm of granulosa cells, whereas no expression was detected in the nucleus. Granulosa cells exhibited a linear correlation between TFAM mRNA and TFAM protein expression. The study participants were divided into three groups using the ternary method based on relative TFAM mRNA expression thresholds of 33% and 76%: the low-expression group (n = 30), the moderate-expression group (n = 27), and the high-expression group (n = 28). When compared to the other two groups, the moderate expression group exhibited a significantly higher egg utilization rate, 2 pronucleus rate, fertilization rate, and clinical pregnancy rate (P < 0.05). Conclusion: TFAM was detected in the cytoplasm of human ovarian granulosa cells. Women with moderate TFAM expression demonstrate enhanced outcomes in IVF. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| معلومات مُعتمدة: | No.20201117 Youth Science and Technology Project of the Scientific Research Program of the Health Commission of Hebei Province |
| فهرسة مساهمة: | Keywords: Embryo quality; Granulosa cells; In vitro fertilization; Infertility; Mitochondrial transcription factor A; Oocyte cytoplasmic mass |
| المشرفين على المادة: | 0 (mitochondrial transcription factor A) 0 (RNA, Messenger) 0 (DNA-Binding Proteins) 0 (Transcription Factors) 0 (Mitochondrial Proteins) |
| تواريخ الأحداث: | Date Created: 20231211 Date Completed: 20240227 Latest Revision: 20240306 |
| رمز التحديث: | 20240306 |
| مُعرف محوري في PubMed: | PMC10894778 |
| DOI: | 10.1007/s10815-023-03001-9 |
| PMID: | 38079076 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-7330 |
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| DOI: | 10.1007/s10815-023-03001-9 |