دورية أكاديمية
Molecular characterization and antimicrobial activity of cecropin family in Hermetia illucens.
| العنوان: | Molecular characterization and antimicrobial activity of cecropin family in Hermetia illucens. |
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| المؤلفون: | Peng J; Translational Medicine Research Center, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China; Guizhou Institute of Precision Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, 550009, Guizhou, People's Republic of China; Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., Li L; Guizhou Institute of Precision Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, 550009, Guizhou, People's Republic of China; Department of Intensive Care Unit, Affiliated Hospital of Guizhou Medical University, Guiyang, 550009, Guizhou, People's Republic of China., Wan Y; Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., Yang Y; Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., An X; Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., Yuan K; Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., Qiu Z; Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., Jiang Y; Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., Guo G; Translational Medicine Research Center, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China., Shen F; Guizhou Institute of Precision Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, 550009, Guizhou, People's Republic of China; Department of Intensive Care Unit, Affiliated Hospital of Guizhou Medical University, Guiyang, 550009, Guizhou, People's Republic of China. Electronic address: doctorshenfeng@gmc.edu.cn., Liang G; Translational Medicine Research Center, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China; Department of Cardiac Surgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, 550009, Guizhou, People's Republic of China. Electronic address: liangguiyou@gmc.edu.cn. |
| المصدر: | Developmental and comparative immunology [Dev Comp Immunol] 2024 Mar; Vol. 152, pp. 105111. Date of Electronic Publication: 2023 Dec 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown Ny : Elsevier Science Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004. |
| مواضيع طبية MeSH: | Cecropins*/genetics , Cecropins*/pharmacology , Anti-Infective Agents*/pharmacology , Diptera*, Animals ; Humans ; Escherichia coli ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Insecta ; Microbial Sensitivity Tests |
| مستخلص: | Antimicrobial peptides are potential alternatives to traditional antibiotics in the face of increasing bacterial resistance. Insects possess many antimicrobial peptides and have become a valuable source of novel and highly effective antimicrobial peptides. Hermetia illucens as a resource insect, for example, has the highest number of antimicrobial peptides of any dipteran. However, most antimicrobial peptides, especially cecropin, have not been comprehensively identified and have not been evaluated for their antimicrobial ability. In this study, we analyzed the localization and gene structure of 33 cecropin molecules in the H. illucens genome and evaluated their activity against common human pathogens. The results showed that 32 cecropin molecules were concentrated on 1 chromosome, most with 2 exons. More importantly, most of the cecropins had a good antibacterial effect against Gram-negative bacteria, and were not hemolytic. The minimum inhibitory concentration (MIC) of the cecropin designated H3 against E. coli was 4 μg/mL. The toxicity, killing time kinetics, and anti-biofilm activity of H3 were further investigated and confirmed its antimicrobial ability. Overall, H3 is a potential candidate for the development of new antimicrobials to treat severe infections caused by Gram-negative pathogens such as E. coli. Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial peptides; Cecropin; Hermetia illucens |
| المشرفين على المادة: | 0 (Cecropins) 0 (Anti-Bacterial Agents) 0 (Anti-Infective Agents) |
| تواريخ الأحداث: | Date Created: 20231211 Date Completed: 20240108 Latest Revision: 20240108 |
| رمز التحديث: | 20240108 |
| DOI: | 10.1016/j.dci.2023.105111 |
| PMID: | 38081402 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0089 |
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| DOI: | 10.1016/j.dci.2023.105111 |