دورية أكاديمية
Morphological evaluation of the feline placenta correlates with gene expression of vascular growth factors and receptors†.
| العنوان: | Morphological evaluation of the feline placenta correlates with gene expression of vascular growth factors and receptors†. |
|---|---|
| المؤلفون: | Gudenschwager-Basso EK; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA., Frydman G; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA., Weerakoon S; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA.; Virginia Tech Carilion School of Medicine, Virginia Tech, Blacksburg, VA, USA., Andargachew H; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA., Piltaver CM; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA., Huckle WR; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA. |
| المصدر: | Biology of reproduction [Biol Reprod] 2024 Mar 13; Vol. 110 (3), pp. 569-582. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Champaign, Ill. : Society for the Study of Reproduction |
| مواضيع طبية MeSH: | Vascular Endothelial Growth Factor A*/genetics , Vascular Endothelial Growth Factor A*/metabolism , Placenta*/metabolism, Cats ; Pregnancy ; Animals ; Female ; Vascular Endothelial Growth Factors/analysis ; Vascular Endothelial Growth Factors/genetics ; Vascular Endothelial Growth Factors/metabolism ; Endothelial Cells ; Placenta Growth Factor/genetics ; Placenta Growth Factor/metabolism ; Vascular Endothelial Growth Factor Receptor-1/genetics ; Vascular Endothelial Growth Factor Receptor-1/metabolism ; Intercellular Signaling Peptides and Proteins/metabolism ; Vascular Endothelial Growth Factor Receptor-2/metabolism ; Gene Expression |
| مستخلص: | Placental angiogenesis is critical for normal development. Angiogenic factors and their receptors are key regulators of this process. Dysregulated placental vascular development is associated with pregnancy complications. Despite their importance, vascular growth factor expression has not been thoroughly correlated with placental morphologic development across gestation in cats. We postulate that changes in placental vessel morphology can be appreciated as consequences of dynamic expression of angiogenic signaling agents. Here, we characterized changes in placental morphology alongside expression analysis of angiogenic factor splice variants and receptors throughout pregnancy in domestic shorthair cats. We observed increased vascular and lamellar density in the lamellar zone during mid-pregnancy. Immunohistochemical analysis localized the vascular endothelial growth factor A (VEGF-A) receptor KDR to endothelial cells of the maternal and fetal microvasculatures. PlGF and its principal receptor Flt-1 were localized to the trophoblasts and fetal vasculature. VEGF-A was found in trophoblast cells and associated with endothelial cells. We detected expression of two Plgf splice variants and four Vegf-a variants. Quantitative real-time polymerase chain reaction analysis showed upregulation of mRNAs encoding pan Vegf-a and all Vegf-a splice forms at gestational days 30-35. Vegf-A showed a marked relative increase in expression during mid-pregnancy, consistent with the pro-angiogenic changes seen in the lamellar zone at days 30-35. Flt-1 was upregulated during late pregnancy. Plgf variants showed stable expression during the first two-thirds of pregnancy, followed by a marked increase toward term. These findings revealed specific spatiotemporal expression patterns of VEGF-A family members consistent with pivotal roles during normal placental development. (© The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | Virginia Maryland College of Veterinary Medicine; Virginia-Maryland College of Veterinary Medicine |
| فهرسة مساهمة: | Keywords: angiogenesis; cat; growth factors; placenta; placental growth factor; vascular endothelial growth factor |
| المشرفين على المادة: | 0 (Vascular Endothelial Growth Factor A) 0 (Vascular Endothelial Growth Factors) 144589-93-5 (Placenta Growth Factor) EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1) 0 (Intercellular Signaling Peptides and Proteins) EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2) |
| تواريخ الأحداث: | Date Created: 20231213 Date Completed: 20240318 Latest Revision: 20240318 |
| رمز التحديث: | 20240318 |
| DOI: | 10.1093/biolre/ioad167 |
| PMID: | 38092011 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1529-7268 |
|---|---|
| DOI: | 10.1093/biolre/ioad167 |