دورية أكاديمية
IgE and IgG4 epitopes of the peanut allergens shift following oral immunotherapy.
| العنوان: | IgE and IgG4 epitopes of the peanut allergens shift following oral immunotherapy. |
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| المؤلفون: | Rambo IM; United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, United States., Kronfel CM; United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, United States., Rivers AR; United States Department of Agriculture, Agricultural Research Service -Genomics and Bioinformatics Research Unit, Gainesville, FL, United States., Swientoniewski LT; United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, United States., McBride JK; United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, United States., Cheng H; United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, United States., Simon RJ; Aimmune Nestlé Health Science, Brisbane, CA, United States., Ryan R; Aimmune Nestlé Health Science, Brisbane, CA, United States., Tilles SA; Aimmune Nestlé Health Science, Brisbane, CA, United States., Nesbit JB; Delgado Community College, New Orleans, LA, United States., Kulis MD; Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill, NC, United States., Hurlburt BK; United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, United States., Maleki SJ; United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, United States. |
| المصدر: | Frontiers in allergy [Front Allergy] 2023 Nov 29; Vol. 4, pp. 1279290. Date of Electronic Publication: 2023 Nov 29 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 9918227355906676 Publication Model: eCollection Cited Medium: Internet ISSN: 2673-6101 (Electronic) Linking ISSN: 26736101 NLM ISO Abbreviation: Front Allergy Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne, Switzerland : Frontiers Media S.A., [2020]- |
| مستخلص: | Background: Oral immunotherapy (OIT) with peanut ( Arachis hypogaea ) allergen powder-dnfp (PTAH; Aimmune Therapeutics) is an FDA-approved treatment to desensitize peanut allergic participants. Objective: Here we assessed shifts in IgE and IgG4 binding to peanut allergens and their epitopes recognized by United States (US) peanut allergic participants ( n = 20) enrolled in phase 3 PTAH OIT clinical trials. Methods: Pre- and post- trial participant sera were collected approximately 12 months apart and tested for IgE binding to intact peanut proteins via ImmunoCAP ISAC immunoassays. IgE and IgG4 linear epitopes were identified based on binding to synthetic overlapping 15-mer linear peptides of 10 peanut allergens (Ara h 1-11) synthesized on microarray slides. Results: Statistically significant decreases in IgE binding were identified for intact Ara h 2, 3, and 6, and known and newly identified IgE epitopes were shown to exhibit shifts towards IgG4 binding post-OIT, with most linear peptides having increased IgG4 binding after treatment with PTAH. While PTAH does not seem to alter the actual peptide binding patterns significantly after one year of treatment, the IgE and IgG4 binding ratios and intensity are altered. Conclusion: At a population level, the linear IgE and IgG4 epitopes of 10 peanut allergens overlap and that increase in IgG4 with OIT results in displacement of IgE binding to both conformational and linear epitopes. Furthermore, it appears as though the increase in IgG4 is more important to achieve desensitization at the 12-month timepoint than the decrease in IgE. This type of knowledge can be useful in the identification of IgE and IgG4-binding allergen and peptide biomarkers that may indicate desensitization or sustained unresponsiveness of allergic individuals to peanut. Competing Interests: SAT is an employee of Aimmune Nestle Health Science. This study was funded by The United States Department of Agriculture (USDA), Agricultural Research Service and by Aimmune Nestlé Health Science, via a Cooperative Research and Development Agreement (CRADA agreement #58-6054-1-0011). Aimmune Nestlé was involved in performing the OIT study with Palforzia, which is published information, but was not involved in the design and interpretation of this study. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (© 2023 Rambo, Kronfel, Rivers, Swientoniewski, McBride, Cheng, Simon, Ryan, Tilles, Nesbit, Kulis, Hurlburt and Maleki.) |
| فهرسة مساهمة: | Keywords: ISAC; epitope; immunoglobulin; linear peptide; machine learning; microarray; oral immunotherapy; peanut allergy |
| تواريخ الأحداث: | Date Created: 20231214 Latest Revision: 20231214 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10717846 |
| DOI: | 10.3389/falgy.2023.1279290 |
| PMID: | 38093814 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2673-6101 |
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| DOI: | 10.3389/falgy.2023.1279290 |