دورية أكاديمية
Fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise.
العنوان: | Fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise. |
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المؤلفون: | Raue U; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Begue G; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Minchev K; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Jemiolo B; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Gries KJ; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Chambers T; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Rubenstein A; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, United States., Zaslavsky E; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, United States., Sealfon SC; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, United States., Trappe T; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Trappe S; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States. |
المصدر: | Journal of applied physiology (Bethesda, Md. : 1985) [J Appl Physiol (1985)] 2024 Feb 01; Vol. 136 (2), pp. 244-261. Date of Electronic Publication: 2023 Dec 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 8502536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1601 (Electronic) Linking ISSN: 01617567 NLM ISO Abbreviation: J Appl Physiol (1985) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1985- |
مواضيع طبية MeSH: | Muscle Fibers, Fast-Twitch*/physiology , Muscle Fibers, Slow-Twitch*/physiology, Male ; Humans ; Transcriptome ; Exercise/physiology ; Muscle Fibers, Skeletal ; Muscle, Skeletal/physiology |
مستخلص: | We investigated fast and slow muscle fiber transcriptome exercise dynamics among three groups of men: lifelong exercisers (LLE, n = 8, 74 ± 1 yr), old healthy nonexercisers (OH, n = 9, 75 ± 1 yr), and young exercisers (YE, n = 8, 25 ± 1 yr). On average, LLE had exercised ∼4 day·wk -1 for ∼8 h·wk -1 over 53 ± 2 years. Muscle biopsies were obtained pre- and 4 h postresistance exercise (3 × 10 knee extensions at 70% 1-RM). Fast and slow fiber size and function were assessed preexercise with fast and slow RNA-seq profiles examined pre- and postexercise. LLE fast fiber size was similar to OH, which was ∼30% smaller than YE ( P < 0.05) with contractile function variables among groups, resulting in lower power in LLE ( P < 0.05). LLE slow fibers were ∼30% larger and more powerful compared with YE and OH ( P < 0.05). At the transcriptome level, fast fibers were more responsive to resistance exercise compared with slow fibers among all three cohorts ( P < 0.05). Exercise induced a comprehensive biological response in fast fibers ( P < 0.05) including transcription, signaling, skeletal muscle cell differentiation, and metabolism with vast differences among the groups. Fast fibers from YE exhibited a growth and metabolic signature, with LLE being primarily metabolic, and OH showing a strong stress-related response. In slow fibers, only LLE exhibited a biological response to exercise ( P < 0.05), which was related to ketone and lipid metabolism. The divergent exercise transcriptome signatures provide novel insight into the molecular regulation in fast and slow fibers with age and exercise and suggest that the ∼5% weekly exercise time commitment of the lifelong exercisers provided a powerful investment for fast and slow muscle fiber metabolic health at the molecular level. NEW & NOTEWORTHY This study provides the first insights into fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise. The fast fibers were more responsive to exercise with divergent transcriptome signatures among young exercisers (growth and metabolic), lifelong exercisers (metabolic), and old healthy nonexercisers (stress). Only lifelong exercisers had a biological response in slow fibers (metabolic). These data provide novel insights into fast and slow muscle fiber health at the molecular level with age and exercise. |
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معلومات مُعتمدة: | R01 AG038576 United States AG NIA NIH HHS; R01-AG38576 HHS | National Institutes of Health (NIH) |
فهرسة مساهمة: | Keywords: aging; exercise; masters athletes; skeletal muscle; transcriptome |
تواريخ الأحداث: | Date Created: 20231214 Date Completed: 20240126 Latest Revision: 20240704 |
رمز التحديث: | 20240704 |
مُعرف محوري في PubMed: | PMC11219013 |
DOI: | 10.1152/japplphysiol.00442.2023 |
PMID: | 38095016 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1522-1601 |
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DOI: | 10.1152/japplphysiol.00442.2023 |