دورية أكاديمية
Fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise.
| العنوان: | Fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise. |
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| المؤلفون: | Raue U; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Begue G; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Minchev K; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Jemiolo B; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Gries KJ; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Chambers T; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Rubenstein A; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, United States., Zaslavsky E; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, United States., Sealfon SC; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, United States., Trappe T; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States., Trappe S; Human Performance Laboratory, Ball State University, Muncie, Indiana, United States. |
| المصدر: | Journal of applied physiology (Bethesda, Md. : 1985) [J Appl Physiol (1985)] 2024 Feb 01; Vol. 136 (2), pp. 244-261. Date of Electronic Publication: 2023 Dec 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 8502536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1601 (Electronic) Linking ISSN: 01617567 NLM ISO Abbreviation: J Appl Physiol (1985) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1985- |
| مواضيع طبية MeSH: | Muscle Fibers, Fast-Twitch*/physiology , Muscle Fibers, Slow-Twitch*/physiology, Male ; Humans ; Transcriptome ; Exercise/physiology ; Muscle Fibers, Skeletal ; Muscle, Skeletal/physiology |
| مستخلص: | We investigated fast and slow muscle fiber transcriptome exercise dynamics among three groups of men: lifelong exercisers (LLE, n = 8, 74 ± 1 yr), old healthy nonexercisers (OH, n = 9, 75 ± 1 yr), and young exercisers (YE, n = 8, 25 ± 1 yr). On average, LLE had exercised ∼4 day·wk -1 for ∼8 h·wk -1 over 53 ± 2 years. Muscle biopsies were obtained pre- and 4 h postresistance exercise (3 × 10 knee extensions at 70% 1-RM). Fast and slow fiber size and function were assessed preexercise with fast and slow RNA-seq profiles examined pre- and postexercise. LLE fast fiber size was similar to OH, which was ∼30% smaller than YE ( P < 0.05) with contractile function variables among groups, resulting in lower power in LLE ( P < 0.05). LLE slow fibers were ∼30% larger and more powerful compared with YE and OH ( P < 0.05). At the transcriptome level, fast fibers were more responsive to resistance exercise compared with slow fibers among all three cohorts ( P < 0.05). Exercise induced a comprehensive biological response in fast fibers ( P < 0.05) including transcription, signaling, skeletal muscle cell differentiation, and metabolism with vast differences among the groups. Fast fibers from YE exhibited a growth and metabolic signature, with LLE being primarily metabolic, and OH showing a strong stress-related response. In slow fibers, only LLE exhibited a biological response to exercise ( P < 0.05), which was related to ketone and lipid metabolism. The divergent exercise transcriptome signatures provide novel insight into the molecular regulation in fast and slow fibers with age and exercise and suggest that the ∼5% weekly exercise time commitment of the lifelong exercisers provided a powerful investment for fast and slow muscle fiber metabolic health at the molecular level. NEW & NOTEWORTHY This study provides the first insights into fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise. The fast fibers were more responsive to exercise with divergent transcriptome signatures among young exercisers (growth and metabolic), lifelong exercisers (metabolic), and old healthy nonexercisers (stress). Only lifelong exercisers had a biological response in slow fibers (metabolic). These data provide novel insights into fast and slow muscle fiber health at the molecular level with age and exercise. |
| References: | J Gerontol A Biol Sci Med Sci. 2015 Feb;70(2):232-46. (PMID: 24846769) Semin Cell Dev Biol. 2022 Aug;128:112-119. (PMID: 34229948) Aging Cell. 2018 Apr;17(2):. (PMID: 29517834) J Appl Physiol (1985). 2010 May;108(5):1410-6. (PMID: 20203068) J Appl Physiol (1985). 2021 Mar 1;130(3):545-561. (PMID: 33356984) Biochem Biophys Res Commun. 2004 Jul 30;320(3):1043-50. (PMID: 15240153) Can J Appl Physiol. 1993 Dec;18(4):331-58. (PMID: 8275048) Med Sci Sports Exerc. 2000 Dec;32(12):48-56. (PMID: 11224794) Commun Biol. 2022 Oct 22;5(1):1121. (PMID: 36273106) Bioinformatics. 2014 Apr 1;30(7):923-30. (PMID: 24227677) Am J Physiol Cell Physiol. 2020 Dec 1;319(6):C1158-C1162. (PMID: 32997515) J Neurol Sci. 1988 Apr;84(2-3):275-94. (PMID: 3379447) J Appl Physiol (1985). 1993 Nov;75(5):2125-33. (PMID: 8307869) J Appl Physiol (1985). 2005 May;98(5):1745-52. (PMID: 15618316) Am J Physiol Endocrinol Metab. 2006 Jun;290(6):E1245-52. (PMID: 16434552) Anal Biochem. 1983 Mar;129(2):277-87. (PMID: 6189421) Exerc Sport Sci Rev. 2023 Apr 1;51(2):51-56. (PMID: 36722844) J Physiol. 2003 Oct 1;552(Pt 1):47-58. (PMID: 12837929) Anal Biochem. 2012 Jun 15;425(2):175-82. (PMID: 22469996) Genome Biol. 2014;15(12):550. (PMID: 25516281) Free Radic Biol Med. 2016 Sep;98:131-143. (PMID: 26876650) Int J Sports Med. 2022 Jul;43(8):661-678. (PMID: 35122228) Skelet Muscle. 2015 Oct 09;5:35. (PMID: 26457177) Med Sci Sports Exerc. 2007 Nov;39(11):1989-96. (PMID: 17986907) Front Immunol. 2018 Nov 20;9:2664. (PMID: 30515158) J Appl Physiol (1985). 2010 Jun;108(6):1501-9. (PMID: 20299622) PLoS One. 2014 Sep 30;9(9):e108547. (PMID: 25268477) Proc R Soc Lond B Biol Sci. 1949 Jun 23;136(883):195-211. (PMID: 18152150) Acta Physiol Scand. 1990 Sep;140(1):41-54. (PMID: 2275404) Cell Metab. 2013 Feb 5;17(2):162-84. (PMID: 23395166) J Appl Physiol (1985). 1989 Jan;66(1):465-75. (PMID: 2917951) Am J Physiol Regul Integr Comp Physiol. 2009 Nov;297(5):R1452-9. (PMID: 19692660) Science. 2020 Sep 11;369(6509):1318-1330. (PMID: 32913098) Metabol Open. 2020 Nov 23;9:100068. (PMID: 33364594) J Appl Physiol (1985). 2013 Sep 1;115(5):667-79. (PMID: 23766501) Physiol Genomics. 2003 Jul 07;14(2):149-59. (PMID: 12783983) J Appl Physiol (1985). 2017 Apr 1;122(4):952-967. (PMID: 28057818) J Physiol. 2021 Jul;599(14):3549-3565. (PMID: 34036579) J Appl Physiol (1985). 2007 Nov;103(5):1744-51. (PMID: 17823296) J Appl Physiol (1985). 2006 Nov;101(5):1442-50. (PMID: 16840578) Am J Physiol Regul Integr Comp Physiol. 2008 Jul;295(1):R273-80. (PMID: 18448613) J Appl Physiol (1985). 2008 Aug;105(2):629-36. (PMID: 18535124) Mech Ageing Dev. 2000 Dec 1;120(1-3):45-56. (PMID: 11087903) J Appl Physiol (1985). 2020 Dec 1;129(6):1493-1504. (PMID: 33054657) Am J Med. 2006 Jun;119(6):526.e9-17. (PMID: 16750969) J Gen Physiol. 2023 Jul 3;155(7):. (PMID: 37227464) J Appl Physiol (1985). 2009 May;106(5):1611-7. (PMID: 19246651) Med Sci Sports Exerc. 2004 Jan;36(1):74-8. (PMID: 14707771) Am J Hum Genet. 2019 Jun 6;104(6):1223-1232. (PMID: 31130282) Mech Ageing Dev. 2013 Nov-Dec;134(11-12):531-40. (PMID: 24287006) Med Sci Sports Exerc. 2016 Mar;48(3):472-80. (PMID: 26460630) J Appl Physiol (1985). 2020 Feb 1;128(2):368-378. (PMID: 31829806) J Appl Physiol (1985). 2015 Jun 15;118(12):1460-6. (PMID: 25749440) J Appl Physiol (1985). 2000 Feb;88(2):627-33. (PMID: 10658030) J Appl Physiol (1985). 2019 Dec 1;127(6):1710-1719. (PMID: 31670601) J Physiol. 2010 Sep 15;588(Pt 18):3567-92. (PMID: 20660569) J Appl Physiol (1985). 2000 Jul;89(1):143-52. (PMID: 10904046) J Physiol. 2003 Mar 15;547(Pt 3):977-87. (PMID: 12562918) J Electromyogr Kinesiol. 1999 Apr;9(2):87-95. (PMID: 10098709) Nat Commun. 2021 Apr 1;12(1):2014. (PMID: 33795677) Cell Metab. 2017 Mar 7;25(3):581-592. (PMID: 28273480) Med Sci Sports Exerc. 2003 Nov;35(11):1875-81. (PMID: 14600553) Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886) Cell Rep. 2017 Jun 13;19(11):2396-2409. (PMID: 28614723) J Appl Physiol (1985). 2012 Nov;113(9):1495-504. (PMID: 22984247) J Appl Physiol (1985). 2007 Nov;103(5):1752-6. (PMID: 17823300) Geroscience. 2022 Aug;44(4):1925-1940. (PMID: 35325353) J Physiol. 2004 Jun 1;557(Pt 2):501-13. (PMID: 15064323) Cell Rep. 2020 Jun 23;31(12):107808. (PMID: 32579934) J Appl Physiol (1985). 2015 Mar 1;118(5):569-78. (PMID: 25539934) Am J Physiol. 1999 Jul;277(1):E135-43. (PMID: 10409137) J Physiol. 1979 Jul;292:177-92. (PMID: 314975) J Physiol. 2022 Dec;600(23):5005-5026. (PMID: 36268622) J Appl Physiol (1985). 1990 May;68(5):1896-901. (PMID: 2361892) J Physiol. 1979 Jun;291:143-59. (PMID: 314510) J Appl Physiol (1985). 2020 Jan 1;128(1):87-99. (PMID: 31751180) J Appl Physiol (1985). 2018 Nov 1;125(5):1636-1645. (PMID: 30161005) J Appl Physiol (1985). 1995 Mar;78(3):823-9. (PMID: 7775325) Physiol Genomics. 2010 Feb 4;40(3):141-9. (PMID: 19903761) J Gerontol A Biol Sci Med Sci. 2002 May;57(5):M326-32. (PMID: 11983728) Biochem Biophys Res Commun. 2015 Aug 14;464(1):208-13. (PMID: 26102030) Sci Rep. 2020 Jan 14;10(1):229. (PMID: 31937892) J Appl Physiol (1985). 2020 Dec 1;129(6):1483-1492. (PMID: 32969782) J Gerontol A Biol Sci Med Sci. 2009 Nov;64(11):1183-9. (PMID: 19622801) J Appl Physiol (1985). 2012 May;112(10):1625-36. (PMID: 22302958) J Neurol Sci. 1992 Feb;107(2):250-1. (PMID: 1564525) J Appl Physiol (1985). 1992 Sep;73(3):812-6. (PMID: 1400042) Nat Methods. 2019 Jul;16(7):607-610. (PMID: 31249421) Am J Physiol Endocrinol Metab. 2022 Mar 1;322(3):E260-E277. (PMID: 35068187) |
| معلومات مُعتمدة: | R01 AG038576 United States AG NIA NIH HHS; R01-AG38576 HHS | National Institutes of Health (NIH) |
| فهرسة مساهمة: | Keywords: aging; exercise; masters athletes; skeletal muscle; transcriptome |
| تواريخ الأحداث: | Date Created: 20231214 Date Completed: 20240126 Latest Revision: 20240704 |
| رمز التحديث: | 20240704 |
| مُعرف محوري في PubMed: | PMC11219013 |
| DOI: | 10.1152/japplphysiol.00442.2023 |
| PMID: | 38095016 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1601 |
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| DOI: | 10.1152/japplphysiol.00442.2023 |