دورية أكاديمية
A plasmid containing the human metallothionein-II gene selectively distinguishes trivalent lanthanum from several divalent heavy metal cations during monoclonal antibody-assisted agarose gel electrophoresis.
| العنوان: | A plasmid containing the human metallothionein-II gene selectively distinguishes trivalent lanthanum from several divalent heavy metal cations during monoclonal antibody-assisted agarose gel electrophoresis. |
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| المؤلفون: | Wooten DC; Department of Anatomy, Pathology Section, Sam Houston State University, College of Osteopathic Medicine, Conroe, TX, USA. |
| المصدر: | Toxicology and industrial health [Toxicol Ind Health] 2024 Jan-Feb; Vol. 40 (1-2), pp. 69-74. Date of Electronic Publication: 2023 Dec 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: SAGE Publications Country of Publication: England NLM ID: 8602702 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-0393 (Electronic) Linking ISSN: 07482337 NLM ISO Abbreviation: Toxicol Ind Health Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : SAGE Publications Original Publication: Princeton, N.J. : Princeton Scientific Pub. Co. |
| مواضيع طبية MeSH: | Lanthanoid Series Elements* , Metallothionein*/genetics , Metals, Heavy*, Humans ; Antibodies, Monoclonal/genetics ; Cations ; Electrophoresis, Agar Gel ; Lanthanum ; Plasmids/genetics ; Sepharose |
| مستخلص: | Trivalent lanthanide ions are known for their ability to interact with calcium-binding sites in various proteins. There is a need to assess the bioavailability of lanthanides and other heavy metals introduced into the body as components of implants or as contrast agents. This study aimed to develop a method to address bioavailability and/or presence of trivalent lanthanide ions by examining electrophoretic mobility in an agarose gel of a plasmid harboring the human metallothionein-II gene ( hMT-II ). Mobility of the plasmid was specifically altered by a monoclonal antibody raised against the zinc-binding transcription factor that controls the activity of the hMT-II gene. This study showed that the plasmid acquired a lanthanide-specific mobility pattern that allowed the presence of lanthanide ions to be readily determined in a 0.8% agarose gel. These findings suggest that this plasmid/monoclonal antibody combination under selected conditions may be useful in industrial, environmental, and biomedical settings to identify, separate, or capture lanthanide ions in complex mixtures that contain an array of metal ions. Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. |
| فهرسة مساهمة: | Keywords: Electrophoresis; agarose; cobalt; gel; heavy metal; lanthanide; lanthanum; mobility; nickel; plasmid; zinc |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Cations) 0 (Lanthanoid Series Elements) 6I3K30563S (Lanthanum) 9038-94-2 (Metallothionein) 0 (Metals, Heavy) 9012-36-6 (Sepharose) |
| تواريخ الأحداث: | Date Created: 20231214 Date Completed: 20240205 Latest Revision: 20240205 |
| رمز التحديث: | 20240205 |
| DOI: | 10.1177/07482337231222354 |
| PMID: | 38095284 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1477-0393 |
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| DOI: | 10.1177/07482337231222354 |