دورية أكاديمية
Using 13 C enriched acetate in isotope labelling incubation experiments: a note of caution.
| العنوان: | Using 13 C enriched acetate in isotope labelling incubation experiments: a note of caution. |
|---|---|
| المؤلفون: | Leone F; Geotop and Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, Canada., Imfeld A; Geotop and Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, Canada., Mirzaei Y; Geotop and Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, Canada., Gélinas Y; Geotop and Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, Canada. |
| المصدر: | Isotopes in environmental and health studies [Isotopes Environ Health Stud] 2024 Mar; Vol. 60 (1), pp. 66-73. Date of Electronic Publication: 2024 Jan 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 9602611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-2639 (Electronic) Linking ISSN: 10256016 NLM ISO Abbreviation: Isotopes Environ Health Stud Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: [Switzerland?] : Basel, Switzerland ; Langhorne, PA : Published under license by Gordon and Breach Science Publishers ; Distributed by International Publishers Distributor, c1995- |
| مواضيع طبية MeSH: | Carbon* , Acetates*, Carbon Isotopes/analysis ; Isotope Labeling ; Mass Spectrometry |
| مستخلص: | Vapour-phase fumigation with HCl is routinely used to remove inorganic carbon in preparation for the measurement of the concentration and δ 13 C value of organic carbon in a sample using elemental analysis coupled to an isotope ratio mass spectrometer. Acidification of the sample to be analyzed can lead to the loss of low molecular weight conjugate bases as volatile organic acids during the acidification and/or the drying steps following fumigation, through protonation of the conjugate base and volatilization. Such loss could lead to a severe bias in incubation experiments where 13 C-enriched compounds such as acetate are used to trace reaction pathways or metabolites in a cultivation medium or a mesocosm for example. In this work, we enriched a carbonate-free freshwater sediment with 1- 13 C sodium acetate by 5, 10 and 20 ‰ relative to the δ 13 C value of the natural organic carbon of the sediment, and then tested the effects of HCl fumigation, drying at 50 °C and drying at room temperature, alone or in combination, on the measured δ 13 C values. We found that fumigation and drying at 50 °C, alone or in combination, both lead to the loss of the majority of the 13 C-enriched acetate spike. |
| فهرسة مساهمة: | Keywords: Acid fumigation; biogeochemistry; carbon-13; elemental analysis; incubation experiment; isotope labelling; organic carbon; stable isotope tracer technique |
| المشرفين على المادة: | 0 (Carbon Isotopes) 7440-44-0 (Carbon) 0 (Acetates) |
| تواريخ الأحداث: | Date Created: 20231215 Date Completed: 20240201 Latest Revision: 20240201 |
| رمز التحديث: | 20240201 |
| DOI: | 10.1080/10256016.2023.2291460 |
| PMID: | 38097918 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher 
Full Text Finder | تدمد: | 1477-2639 |
|---|---|
| DOI: | 10.1080/10256016.2023.2291460 |