دورية أكاديمية
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The potential mechanism of response to light intensity in energy metabolism mediated by miRNA in Isatis indigotica.

التفاصيل البيبلوغرافية
العنوان: The potential mechanism of response to light intensity in energy metabolism mediated by miRNA in Isatis indigotica.
المؤلفون: Zhao K; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China., Pu Y; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China., Shi H; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China., Guo Q; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China., Su Y; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China., Yang F; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China., Liu C; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China., Du Y; Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing City, Jiangsu Province 210095, PR China.
المصدر: Gene [Gene] 2024 Mar 01; Vol. 897, pp. 148083. Date of Electronic Publication: 2023 Dec 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
مواضيع طبية MeSH: Isatis*/genetics , MicroRNAs*/genetics , MicroRNAs*/metabolism, Light ; Plant Development
مستخلص: Light is the main source of energy for plant growth. Studies have shown that I. indigotica is a light-demanding plant and its yield and various active components are positively correlated with light intensity, but no studies of light intensity affecting energy metabolism in I. indigotica have been reported. Mitochondria are the main site of energy metabolism, and miRNAs are important factors in regulating gene expression, this experiment attempts to study the effects of different light intensities on energy metabolism from the perspective of mitochondria and miRNAs. The results show that the biomass、mitochondrial structural integrity and energy metabolism in I. indigotica were found to be positively correlated with light intensity. Small RNA and transcriptome sequencing identified 241 miRNAs and 36,372 mRNAs, and degradomic technology identified 72 miRNAs targeting 106 mRNAs, among which 12 pairs of miRNA-mRNAs were annotated on mitochondria. Combined with RT-qPCR validation, it was concluded that miR167a-5p positively regulates LETM1 and affects mitochondrial structure, miR400-5p and mIR169m-p3_1ss15CT negatively regulate GRXS15 and CMC4, respectively, affecting SDH and CCO activities, and miR395a-APS4 may affect the utilization of ATP and sulfate assimilation. In summary, the results of this study complement and enrich knowledge of light effects on mitochondria from the perspective of miRNA, while providing guidance for the cultivation of I. indigotica.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Energy metabolism; Isatis indigotica Fortune; Light intensity; Mitochondria; miRNA
المشرفين على المادة: 0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20231215 Date Completed: 20240117 Latest Revision: 20240117
رمز التحديث: 20240117
DOI: 10.1016/j.gene.2023.148083
PMID: 38101709
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0038
DOI:10.1016/j.gene.2023.148083