دورية أكاديمية
The Soft Nanodots as Fluorescent Probes for Cell Imaging: Analysis of Cell and Spheroid Penetration Behavior of Single Chain Polymer Dots.
العنوان: | The Soft Nanodots as Fluorescent Probes for Cell Imaging: Analysis of Cell and Spheroid Penetration Behavior of Single Chain Polymer Dots. |
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المؤلفون: | Yucel M; Department of Chemistry and Pharmacy, Friedrich-Alexander Universität Erlangen-Nürnberg, 91058, Erlangen, Germany.; Department of Bioengineering, Izmir Institute of Technology, İzmir, 35430, Turkey., Onbas R; Department of Bioengineering, Izmir Institute of Technology, İzmir, 35430, Turkey., Arslan Yildiz A; Department of Bioengineering, Izmir Institute of Technology, İzmir, 35430, Turkey., Yildiz UH; Department of Chemistry, Izmir Institute of Technology, İzmir, 35430, Turkey.; Department of Polymer Science and Engineering, Izmir Institute of Technology, İzmir, 35430, Turkey. |
المصدر: | Macromolecular bioscience [Macromol Biosci] 2024 Apr; Vol. 24 (4), pp. e2300402. Date of Electronic Publication: 2024 Jan 10. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101135941 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1616-5195 (Electronic) Linking ISSN: 16165187 NLM ISO Abbreviation: Macromol Biosci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Weinheim, Germany : Wiley-VCH, c2001- |
مواضيع طبية MeSH: | Fluorescent Dyes* , Quantum Dots*, Semiconductors ; Polymers ; Polyelectrolytes ; Solvents |
مستخلص: | This study describes the formation, size control, and penetration behavior of polymer nanodots (Pdots) consisting of single or few chain polythiophene-based conjugated polyelectrolytes (CPEs) via nanophase separation between good solvent and poor solvent of CPE. Though the chain singularity may be associated with dilution nanophase separation suggests that molecules of a good solvent create a thermodynamically driven solvation layer surrounding the CPEs and thereby separating the single chains even in their poor solvents. This statement is therefore corroborated with emission intensity/lifetime, particle size, and scattering intensity of polyelectrolyte in good and poor solvents. Regarding the augmented features, Pdots are implemented into cell imaging studies to understand the nuclear penetration and to differentiate the invasive characteristics of breast cancer cells. The python based red, green, blue (RGB) color analysis depicts that Pdots have more nuclear penetration ability in triple negative breast cancer cells due to the different nuclear morphology in shape and composition and Pdots have penetrated cell membrane as well as extracellular matrix in spheroid models. The current Pdot protocol and its utilization in cancer cell imaging are holding great promise for gene/drug delivery to target cancer cells by explicitly achieving the very first priority of nuclear intake. (© 2024 Wiley‐VCH GmbH.) |
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معلومات مُعتمدة: | 2021IYTE-1-0065 İYTE BAP Project; National Graduate Scholarship Program; 120Z588 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu; 116Z547 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu |
فهرسة مساهمة: | Keywords: conjugated polyelectrolyte; image analysis; nanophase separation; polymer dots; triple negative breast cancer |
المشرفين على المادة: | 0 (Fluorescent Dyes) 0 (Polymers) 0 (Polyelectrolytes) 0 (Solvents) |
تواريخ الأحداث: | Date Created: 20231216 Date Completed: 20240416 Latest Revision: 20240416 |
رمز التحديث: | 20240416 |
DOI: | 10.1002/mabi.202300402 |
PMID: | 38102867 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1616-5195 |
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DOI: | 10.1002/mabi.202300402 |