دورية أكاديمية
Development of 3D melanoma cultures on a hyaluronic acid-based scaffold with synthetic self-assembling peptides: Electroporation enhancement.
| العنوان: | Development of 3D melanoma cultures on a hyaluronic acid-based scaffold with synthetic self-assembling peptides: Electroporation enhancement. |
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| المؤلفون: | Zamuner A; Padova University, Department of Civil, Environmental, and Architectural Engineering, via Marzolo, 9, 35131 Padova, Italy; Padova University, Department of Industrial Engineering, via Marzolo, 9, 35131 Padova, Italy., Dettin M; Padova University, Department of Industrial Engineering, via Marzolo, 9, 35131 Padova, Italy., Dall'Olmo L; Padova University, Department of Surgery Oncology and Gastroenterology, DISCOG. Via Giustiniani 2, 35128 Padova, Italy; Surgical Oncology Unit, Veneto Institute of Oncology (IOV-IRCCS), via Gattamelata 64, 35128 Padova, Italy., Campana LG; Department of Surgery, Manchester University NHS Foundation Trust, Oxford Rd, M13 9WL, Manchester, UK., Mognaschi ME; Pavia University, Department of Electrical, Computer and Biomedical Engineering, via Ferrata, 5, 21100 Padova, Italy., Conconi MT; Padova University, Department of Pharmaceutical and Pharmacological Sciences, via Marzolo, 5, 35131 Padova, Italy., Sieni E; University of Insubria, Department of theoretical and applied sciences, via Dunant, 3, 21100 Varese, Italy. Electronic address: elisabetta.sieni@uninsubria.it. |
| المصدر: | Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2024 Apr; Vol. 156, pp. 108624. Date of Electronic Publication: 2023 Dec 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 100953583 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-562X (Electronic) Linking ISSN: 15675394 NLM ISO Abbreviation: Bioelectrochemistry Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam?] : Elsevier, 2000- |
| مواضيع طبية MeSH: | Hyaluronic Acid*/chemistry , Melanoma*/pathology, Humans ; Electroporation/methods ; Extracellular Matrix ; Collagen/therapeutic use ; Tissue Scaffolds/chemistry ; Tumor Microenvironment |
| مستخلص: | Electrochemotherapy (ECT) with bleomycin is an effective antitumor treatment. Still, researchers are investigating new drugs and electroporation conditions to improve its efficacy. To this aim, in vivo assays are accurate but expensive and ethically questionable. Conversely, in vitro assays, although cheaper and straightforward, do not reflect the architecture of the biological tissue because they lack a tridimensional (3D) structure (as in the case of two-dimensional [2D] in vitro assays) or do not include all the extracellular matrix components (as in the case of 3D in vitro scaffolds). To address this issue, 3D in vitro models have been proposed, including spheroids and hydrogel-based cultures, which require a suitable low-conductive medium to allow cell membrane electroporation. In this study, a synthetic scaffold based on hyaluronic acid (HA) and self-assembling peptides (SAPs; EAbuK), condensed with a Laminin-derived adhesive sequence (IKVAV), is proposed as a reliable alternative. We compare SKMEL28 cells cultured in the HA-EAbuK-IKVAV scaffold to the control (HA only scaffold). Three days after seeding, the culture on the HA-EAbuK-IKVAV scaffold showed collagen production. SKMEL28 cells cultured on the HA-EAbuK-IKVAV scaffold started to be electroporated at 400 V/cm, whereas, at the same electric field intensity, those cultured on HA were not. As a reference, 2D experiments showed that electroporation of SKMEL28 cells starts at 600 V/cm using an electroporation buffer and at 800 V/cm in a culture medium, but with very low efficiency (<50 % of cells electroporated). 3D cultures on HA-EAbuK-IKVAV allowed the simulation of a more reliable microenvironment and may represent a valuable tool for studying electroporation conditions. Using Finite Element Analysis (FEA) to compute the transmembrane potential, we detected the influence of inhomogeneity of the extracellular matrix on electroporation effect. Our 3D cell culture electroporation simulations showed that the transmembrane potential increased when collagen surrounded the cells. Of note, in the collagen-enriched HA-EAbuK-IKVAV scaffold, EP was already improved at lower electric field intensities. This study shows the influence of the extracellular matrix on electric conductivity and electric field distribution on cell membrane electroporation and supports the adoption of more reliable 3D scaffolds in experimental electroporation studies. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 9004-61-9 (Hyaluronic Acid) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20231217 Date Completed: 20240115 Latest Revision: 20240115 |
| رمز التحديث: | 20240115 |
| DOI: | 10.1016/j.bioelechem.2023.108624 |
| PMID: | 38104458 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-562X |
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| DOI: | 10.1016/j.bioelechem.2023.108624 |