دورية أكاديمية
The Effect of the cAMP Signaling Pathway on HTR8/SV-Neo Cell Line Proliferation, Invasion, and Migration After Treatment with Forskolin.
العنوان: | The Effect of the cAMP Signaling Pathway on HTR8/SV-Neo Cell Line Proliferation, Invasion, and Migration After Treatment with Forskolin. |
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المؤلفون: | Sun C; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University), Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, 3 Longhua Road, Haikou, Hainan, 570102, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' by the Ministry of Science and Technology of China, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Mei J; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University), Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, 3 Longhua Road, Haikou, Hainan, 570102, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' by the Ministry of Science and Technology of China, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Yi H; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University), Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, 3 Longhua Road, Haikou, Hainan, 570102, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' by the Ministry of Science and Technology of China, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Song M; Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Ma Y; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University), Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, 3 Longhua Road, Haikou, Hainan, 570102, China. mayanlinma@hotmail.com.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. mayanlinma@hotmail.com.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' by the Ministry of Science and Technology of China, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. mayanlinma@hotmail.com.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. mayanlinma@hotmail.com., Huang Y; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University), Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, 3 Longhua Road, Haikou, Hainan, 570102, China. huangyh4180@hainmc.edu.cn.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. huangyh4180@hainmc.edu.cn.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' by the Ministry of Science and Technology of China, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. huangyh4180@hainmc.edu.cn.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. huangyh4180@hainmc.edu.cn. |
المصدر: | Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 May; Vol. 31 (5), pp. 1268-1277. Date of Electronic Publication: 2023 Dec 18. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101291249 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1933-7205 (Electronic) Linking ISSN: 19337191 NLM ISO Abbreviation: Reprod Sci Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2020- : [New York] : Springer Original Publication: Thousand Oaks, Calif. : Sage |
مواضيع طبية MeSH: | Cell Movement*/drug effects , Colforsin*/pharmacology , Cell Proliferation*/drug effects , Cyclic AMP*/metabolism , Trophoblasts*/metabolism , Trophoblasts*/drug effects , Signal Transduction*/drug effects, Humans ; Cell Line ; Female ; Pregnancy ; Epithelial-Mesenchymal Transition/drug effects ; Pre-Eclampsia/metabolism ; Pre-Eclampsia/drug therapy ; Pre-Eclampsia/pathology |
مستخلص: | Pre-eclampsia (PE) is thought to be related to placental dysfunction, particularly poor extravillous trophoblast (EVT) invasion and migration abilities. However, the pathogenic mechanism is not fully understood. This article describes the impact of the cyclic adenosine monophosphate(cAMP) signaling pathway on EVT behavior, focusing on EVT proliferation, invasion, and migration. Here, we used the HTR8/SV-neo cell line to study human EVT function in vitro. HTR8/SV-neo cells were treated with different concentrations of forskolin (cAMP pathway-specific agonist) to alter intracellular cAMP levels, and dimethyl sulfoxide (DMSO) was used as the control. First, a cAMP assay was performed to measure the cAMP concentration in HTR8/SV-neo cells treated with different forskolin concentrations, and cell proliferation was assessed by constructing cell growth curves and assessing colony formation. Cell invasion and migration were observed by Transwell experiments, and intracellular epithelial-mesenchymal transition (EMT) marker expression was evaluated by quantitative real-time polymerase chain reaction (qPCR) and Western blotting (WB). According to our research, the intracellular cAMP levels in HTR8/SV-neo cells were increased in a dose-dependent manner, and HTR8/SV-neo cell proliferation, invasion and migration were significantly enhanced. The expression of EMT and angiogenesis markers was upregulated. Additionally, with the increase in intracellular cAMP levels, the phosphorylation of intracellular mitogen-activated protein kinase (MAPK) signaling pathway components was significantly increased. These results suggested that the cAMP signaling pathway promoted the phosphorylation of MAPK signaling components, thus enhancing EVT functions, including proliferation, invasion, and migration, and to a certain extent, providing a novel direction for the treatment of PE patients. (© 2023. The Author(s), under exclusive licence to Society for Reproductive Investigation.) |
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معلومات مُعتمدة: | ZDKJ2021037 Major Science and Technology Project of Hainan Province; 81960283 National Natural Science Foundation of China; 82072880 National Natural Science Foundation of China |
فهرسة مساهمة: | Keywords: Cell function; Extravillous trophoblast; MAPK signaling pathway; Pre-eclampsia; cAMP signaling pathway |
المشرفين على المادة: | 1F7A44V6OU (Colforsin) E0399OZS9N (Cyclic AMP) |
تواريخ الأحداث: | Date Created: 20231219 Date Completed: 20240513 Latest Revision: 20240513 |
رمز التحديث: | 20240514 |
DOI: | 10.1007/s43032-023-01396-5 |
PMID: | 38110819 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1933-7205 |
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DOI: | 10.1007/s43032-023-01396-5 |