دورية أكاديمية
Reference genes for gene expression profiling in mouse models of Listeria monocytogenes infection.
| العنوان: | Reference genes for gene expression profiling in mouse models of Listeria monocytogenes infection. |
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| المؤلفون: | Tavares LS; Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil., Oliveira-Silva RL; Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil., Moura MT; Departamento de Biologia Celular e Molecular, Centro de Biotecnologia, Campus I, Universidade Federal da Paraíba, João Pessoa, PB, Brasil., da Silva JB; Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil., Benko-Iseppon AM; Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil., Lima-Filho JV; Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil. |
| المصدر: | BioTechniques [Biotechniques] 2024 Mar; Vol. 76 (3), pp. 104-113. Date of Electronic Publication: 2023 Dec 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Future Science Country of Publication: England NLM ID: 8306785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1940-9818 (Electronic) Linking ISSN: 07366205 NLM ISO Abbreviation: Biotechniques Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, UK : Future Science Original Publication: [Natick, MA : Eaton Pub. Co., c1983- |
| مواضيع طبية MeSH: | Listeria monocytogenes*/genetics, Animals ; Mice ; Real-Time Polymerase Chain Reaction ; Gene Expression Profiling ; Microarray Analysis ; Cytokines/genetics ; Reference Standards |
| مستخلص: | RT-qPCR dissects transcription-based processes but requires reference genes (RGs) for data normalization. This study prospected RGs for mouse macrophages (pMØ) and spleen infected with Listeria monocytogenes . The pMØ were infected in vitro with L. monocytogenes or vehicle for 4 h. Mice were injected with L. monocytogenes (or vehicle) and euthanized 24 h post-injection. The RGs came from a multispecies primer set, from the literature or designed here. The RG ranking relied on GeNorm, NormFinder, BestKeeper, Delta-CT and RefFinder. B2m - H3f3a - Ppia were the most stable RGs for pMØ, albeit RG indexes fine-tuned estimations of cytokine relative expression. Actβ-Ubc - Ppia were the best RGs for spleen but modestly impacted the cytokine relative expression. Hence, mouse models of L. monocytogenes require context-specific RGs for RT-qPCR, thus reinforcing its paramount contribution to accurate gene expression profiling. |
| معلومات مُعتمدة: | Code 001, PROAP/2019 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
| فهرسة مساهمة: | Keywords: RT-qPCR; cytokine; housekeeping genes; immune response; internal control genes; listeriosis; macrophages; real-time qPCR; relative expression; spleen |
| المشرفين على المادة: | 0 (Cytokines) |
| تواريخ الأحداث: | Date Created: 20231219 Date Completed: 20240319 Latest Revision: 20240319 |
| رمز التحديث: | 20240319 |
| DOI: | 10.2144/btn-2023-0063 |
| PMID: | 38112054 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1940-9818 |
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| DOI: | 10.2144/btn-2023-0063 |