| المؤلفون: |
Pham TM; Dalat Nuclear Research Institute, 01 Nguyen Tu Luc, Dalat, 670000, Vietnam. sciminh@gmail.com., Cao DV; Dalat Nuclear Research Institute, 01 Nguyen Tu Luc, Dalat, 670000, Vietnam. sciminh@gmail.com., Dang HHQ; Dalat Nuclear Research Institute, 01 Nguyen Tu Luc, Dalat, 670000, Vietnam. sciminh@gmail.com., Mai PMT; Dalat Nuclear Research Institute, 01 Nguyen Tu Luc, Dalat, 670000, Vietnam. sciminh@gmail.com., Nguyen TB; Dalat Nuclear Research Institute, 01 Nguyen Tu Luc, Dalat, 670000, Vietnam. sciminh@gmail.com., Dinh NBN; Dalat Nuclear Research Institute, 01 Nguyen Tu Luc, Dalat, 670000, Vietnam. sciminh@gmail.com., Nguyen TKG; Dalat Nuclear Research Institute, 01 Nguyen Tu Luc, Dalat, 670000, Vietnam. sciminh@gmail.com., Le TMH; Nuclear Training Center, Vietnam Atomic Energy Institute, 140 Nguyen Tuan Street, Thanh Xuan District, Hanoi, 110430, Vietnam., Doan VD; The Faculty of Chemical Engineering, Industrial University of Ho Chi Minh City, Ho Chi Minh City, 70000, Vietnam., Nguyen DT; Lam Dong Department of Health, 36 Tran Phu, Lam Dong, 670000, Vietnam., Le VT; Center for Advanced Chemistry, Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang, 550000, Vietnam. levanthuan3@duytan.edu.vn.; Faculty of Natural Sciences, Duy Tan University, 03 Quang Trung, Da Nang, 550000, Vietnam. |
| مستخلص: |
The present study introduces Fe 3 O 4 -coated lapatinib-labeled 153 Sm nanoparticles (denoted as Fe 3 O 4 @lapatinib- 153 Sm) as a promising avenue for advancing breast cancer treatment. The radiolabeled nanoparticles combine various attributes, offering enhanced therapeutic precision. The integration of lapatinib confers therapeutic effects and targeted delivery. The inherent magnetic characteristics of Fe 3 O 4 nanoparticles contribute to improved imaging contrast and targeted localization. Incorporating the gamma-emitting 153 Sm isotope permits single-photon emission computed tomography imaging and radiation dose evaluation, while its beta-emitting nature ensures targeted cancer cell eradication. The synthesis of Fe 3 O 4 @lapatinib- 153 Sm was meticulously optimized by investigating the effects of parameters on radiolabeling efficiency. Physicochemical attributes were scrutinized using several analytical techniques. In-depth in vivo assessment evaluated the biocompatibility, toxicity, and biodistribution in a murine model, illuminating clinical utility. Optimal conditions ( 153 SmCl 3 concentration of 10 mCi mL -1 , pH 7.4, a reaction time of 30 min, and a temperature of 25 °C) achieved >99% labeling efficiency and radiochemical purity. The TEM analysis indicated that the diameter of Fe 3 O 4 @lapatinib- 153 Sm nanoparticles ranged from 10 to 40 nm. Vibrating-sample magnetometry verified their superparamagnetic behaviour with a saturation magnetization of 41.4 emu g -1 . The synthesized radiopharmaceutical exhibited high sterility and in vitro stability. Acute toxicity studies showed the mild effects of Fe 3 O 4 @lapatinib- 153 Sm at a dose of 20 mCi kg -1 , with no observed mortality. Notably, lesions from Fe 3 O 4 @lapatinib- 153 Sm use recovered naturally over time. Radiation doses below 20 mCi kg -1 were recommended for clinical trials. The biodistribution study in BT474 xenograft mice revealed rapid clearance of Fe 3 O 4 @lapatinib- 153 Sm within 48 h. Significant accumulation occurred in the liver, spleen, and tumor tissue, while minimal accumulation was found in other tissues. Future steps involve studying biocorona formation and therapeutic efficacy on tumour models, refining its clinical potential. |
