دورية أكاديمية
Engineering a synthetic energy-efficient formaldehyde assimilation cycle in Escherichia coli.
| العنوان: | Engineering a synthetic energy-efficient formaldehyde assimilation cycle in Escherichia coli. |
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| المؤلفون: | Wu T; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany.; Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt Universität zu Berlin, Institute of Biochemistry, Charitéplatz 1, 10117, Berlin, Germany., Gómez-Coronado PA; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany.; Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany., Kubis A; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany., Lindner SN; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany.; Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt Universität zu Berlin, Institute of Biochemistry, Charitéplatz 1, 10117, Berlin, Germany., Marlière P; TESSSI, The European Syndicate of Synthetic Scientists and Industrialists, 81 rue Réaumur, 75002, Paris, France., Erb TJ; Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany., Bar-Even A; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany., He H; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany. hai.he@mpi-marburg.mpg.de.; Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany. hai.he@mpi-marburg.mpg.de. |
| المصدر: | Nature communications [Nat Commun] 2023 Dec 20; Vol. 14 (1), pp. 8490. Date of Electronic Publication: 2023 Dec 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | Escherichia coli*/genetics , Escherichia coli*/metabolism , Methanol*/metabolism, Formaldehyde/metabolism ; Sarcosine ; Fructose-Bisphosphate Aldolase/metabolism ; Metabolic Engineering |
| مستخلص: | One-carbon (C1) substrates, such as methanol or formate, are attractive feedstocks for circular bioeconomy. These substrates are typically converted into formaldehyde, serving as the entry point into metabolism. Here, we design an erythrulose monophosphate (EuMP) cycle for formaldehyde assimilation, leveraging a promiscuous dihydroxyacetone phosphate dependent aldolase as key enzyme. In silico modeling reveals that the cycle is highly energy-efficient, holding the potential for high bioproduct yields. Dissecting the EuMP into four modules, we use a stepwise strategy to demonstrate in vivo feasibility of the modules in E. coli sensor strains with sarcosine as formaldehyde source. From adaptive laboratory evolution for module integration, we identify key mutations enabling the accommodation of the EuMP reactions with endogenous metabolism. Overall, our study demonstrates the proof-of-concept for a highly efficient, new-to-nature formaldehyde assimilation pathway, opening a way for the development of a methylotrophic platform for a C1-fueled bioeconomy in the future. (© 2023. The Author(s).) |
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| المشرفين على المادة: | Y4S76JWI15 (Methanol) 1HG84L3525 (Formaldehyde) Z711V88R5F (Sarcosine) EC 4.1.2.13 (Fructose-Bisphosphate Aldolase) |
| تواريخ الأحداث: | Date Created: 20231220 Date Completed: 20231222 Latest Revision: 20231223 |
| رمز التحديث: | 20231223 |
| مُعرف محوري في PubMed: | PMC10733421 |
| DOI: | 10.1038/s41467-023-44247-2 |
| PMID: | 38123535 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/s41467-023-44247-2 |