دورية أكاديمية
Electrochemical Nanopipette Sensor for In Vitro/In Vivo Detection of Cu 2+ Ions.
| العنوان: | Electrochemical Nanopipette Sensor for In Vitro/In Vivo Detection of Cu 2+ Ions. |
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| المؤلفون: | Timoshenko RV; National University of Science and Technology (MISIS), Moscow 119049, Russia., Gorelkin PV; National University of Science and Technology (MISIS), Moscow 119049, Russia., Vaneev AN; National University of Science and Technology (MISIS), Moscow 119049, Russia.; Chemistry Department, Lomonosov Moscow State University, Moscow 119991, Russia., Krasnovskaya OO; National University of Science and Technology (MISIS), Moscow 119049, Russia.; Chemistry Department, Lomonosov Moscow State University, Moscow 119991, Russia., Akasov RA; Federal Scientific Research Center 'Crystallography and Photonics' of the Russian Academy of Sciences, Moscow 119333, Russia.; Sechenov First Moscow State Medical University, Moscow 119991, Russia., Garanina AS; National University of Science and Technology (MISIS), Moscow 119049, Russia., Khochenkov DA; N.N. Blokhin National Medical Research Center of Oncology, Moscow 115478, Russia.; Togliatti State University, Togliatti 445020, Russia., Iakimova TM; Chemistry Department, Lomonosov Moscow State University, Moscow 119991, Russia., Klyachko NL; Chemistry Department, Lomonosov Moscow State University, Moscow 119991, Russia., Abakumova TO; Pirogov Russian National Research Medical University, Moscow 117997, Russia., Shashkovskaya VS; Pirogov Russian National Research Medical University, Moscow 117997, Russia., Chaprov KD; Institute of Physiologically Active Compounds at Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry of the Russian Academy of Sciences, Chernogolovka 142432, Russia., Makarov AA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia., Mitkevich VA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia., Takahashi Y; Department of Electronics, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa 920-1192, Japan., Edwards CRW; Department of Medicine, Imperial College London, London W120NN, U.K., Korchev YE; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa 920-1192, Japan.; Department of Medicine, Imperial College London, London W120NN, U.K., Erofeev AS; National University of Science and Technology (MISIS), Moscow 119049, Russia.; Chemistry Department, Lomonosov Moscow State University, Moscow 119991, Russia. |
| المصدر: | Analytical chemistry [Anal Chem] 2024 Jan 09; Vol. 96 (1), pp. 127-136. Date of Electronic Publication: 2023 Dec 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Alzheimer Disease*/diagnosis , Neoplasms*, Mice ; Humans ; Animals ; Copper ; Ions ; Electrochemical Techniques |
| مستخلص: | In vitro/in vivo detection of copper ions is a challenging task but one which is important in the development of new approaches to the diagnosis and treatment of cancer and hereditary diseases such as Alzheimer's, Wilson's, etc. In this paper, we present a nanopipette sensor capable of measuring Cu 2+ ions with a linear range from 0.1 to 10 μM in vitro and in vivo. Using the gold-modified nanopipette sensor with a copper chelating ligand, we evaluated the accumulation ability of the liposomal form of an anticancer Cu-containing complex at three levels of biological organization. First, we detected Cu 2+ ions in a single cell model of human breast adenocarcinoma MCF-7 and in murine melanoma B16 cells. The insertion of the nanoelectrode did not result in leakage of the cell membrane. We then evaluated the distribution of the Cu-complex in MCF-7 tumor spheroids and found that the diffusion-limited accumulation was a function of the depth, typical for 3D culture. Finally, we demonstrated the use of the sensor for Cu 2+ ion detection in the brain of an APP/PS1 transgenic mouse model of Alzheimer's disease and tumor-bearing mice in response to injection (2 mg kg -1 ) of the liposomal form of the anticancer Cu-containing complex. Enhanced stability and selectivity, as well as distinct copper oxidation peaks, confirmed that the developed sensor is a promising tool for testing various types of biological systems. In summary, this research has demonstrated a minimally invasive electrochemical technique with high temporal resolution that can be used for the study of metabolism of copper or copper-based drugs in vitro and in vivo. |
| المشرفين على المادة: | 789U1901C5 (Copper) 0 (Ions) |
| تواريخ الأحداث: | Date Created: 20231221 Date Completed: 20240110 Latest Revision: 20240213 |
| رمز التحديث: | 20240214 |
| DOI: | 10.1021/acs.analchem.3c03337 |
| PMID: | 38126724 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6882 |
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| DOI: | 10.1021/acs.analchem.3c03337 |