دورية أكاديمية
Benzalkonium chloride greatly deteriorates the biological activities of human corneal stroma fibroblasts in a concentration-dependent manner.
| العنوان: | Benzalkonium chloride greatly deteriorates the biological activities of human corneal stroma fibroblasts in a concentration-dependent manner. |
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| المؤلفون: | Umetsu A; Department of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo, Japan., Ida Y; Department of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo, Japan., Sato T; Department of Cardiovascular, Renal and Metabolic Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.; Department of Cellular Physiology and Signal Transduction, Sapporo Medical University School of Medicine, Sapporo, Japan., Furuhashi M; Department of Cardiovascular, Renal and Metabolic Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan., Ohguro H; Department of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo, Japan., Watanabe M; Department of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo, Japan. watanabe@sapmed.ac.jp. |
| المصدر: | Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie [Graefes Arch Clin Exp Ophthalmol] 2024 Jun; Vol. 262 (6), pp. 1847-1855. Date of Electronic Publication: 2023 Dec 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 8205248 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-702X (Electronic) Linking ISSN: 0721832X NLM ISO Abbreviation: Graefes Arch Clin Exp Ophthalmol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer-Verlag, c1982- |
| مواضيع طبية MeSH: | Benzalkonium Compounds*/toxicity , Corneal Stroma*/drug effects , Corneal Stroma*/metabolism , Cell Survival*/drug effects , Preservatives, Pharmaceutical*/toxicity, Humans ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endoplasmic Reticulum Stress/drug effects ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Corneal Keratocytes/drug effects ; Corneal Keratocytes/metabolism ; Real-Time Polymerase Chain Reaction ; RNA, Messenger/genetics ; RNA, Messenger/metabolism |
| مستخلص: | Background: Corneal tissues indirectly obtain nutritional needs and oxygen to maintain their homeostasis, and therefore, benzalkonium chloride (BAC) containing ocular instillations for medical therapy may, in turn, induce toxic effects more than expected in corneal tissues, especially the inside stroma layer. Methods: To evaluate the effects of very low concentrations (10 -8 %, 10 -6 %, or 10 -4 %) of BAC on human corneal stroma, we used two-dimensional (2D) cultures of human corneal stromal fibroblast (HCSF) cells and carried out the following analyses: (1) cell viability measurements, (2) Seahorse cellular bio-metabolism analysis, and (3) the expression of ECM molecules and endoplasmic reticulum (ER) stress-related molecules. Results: In the absence and presence of 10 -8 %, 10 -6 %, or 10 -4 % concentrations of BAC, cell viability deteriorated and this deterioration was dose-dependent. The results showed that maximal mitochondrial respiration was decreased, the mRNA expression of most of ECM proteins was decreased, and ER stress-related molecules were substantially and dose-dependently down-regulated in HCSFs by the BAC treatment. Conclusions: The findings reported herein indicate that the presence of BAC, even at such low concentrations, is capable of causing the deterioration of cellular metabolic functions and negatively affecting the response to ER stress in HCSF cells resulting in a substantially decreased cellular viability. (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Benzalkonium chloride (BAC); Cell viability; Human corneal stroma fibroblasts (HCSFs); Mitochondrial and glycolysis function; Seahorse cellular metabolic analysis |
| تواريخ الأحداث: | Date Created: 20231222 Date Completed: 20240520 Latest Revision: 20240520 |
| رمز التحديث: | 20240521 |
| DOI: | 10.1007/s00417-023-06325-5 |
| PMID: | 38133799 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1435-702X |
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| DOI: | 10.1007/s00417-023-06325-5 |