دورية أكاديمية
Characterization of the chemical composition of different parts of Dolichos lablab L. and revelation of its anti-ulcerative colitis effects by modulating the gut microbiota and host metabolism.
| العنوان: | Characterization of the chemical composition of different parts of Dolichos lablab L. and revelation of its anti-ulcerative colitis effects by modulating the gut microbiota and host metabolism. |
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| المؤلفون: | Gao Y; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China., Huang R; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China., Qiu Y; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China., Liu Y; School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China. Electronic address: liuyi099@163.com., Chen L; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China. Electronic address: chenlei0080@163.com. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2024 Mar 25; Vol. 322, pp. 117629. Date of Electronic Publication: 2023 Dec 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Gastrointestinal Microbiome* , Colitis, Ulcerative*/drug therapy , Dolichos* , Colitis*, Humans ; Animals ; Mice ; RNA, Ribosomal, 16S ; Metabolomics ; Disease Models, Animal ; Dextran Sulfate ; Colon ; Mice, Inbred C57BL |
| مستخلص: | Ethnopharmacological Relevance: Ulcerative colitis (UC) is a non-specific inflammatory disease characterized by long duration and easy relapse. Dolichos lablab L. (DLL) belongs to the family Fabaceae, was listed in a famous Chinese medical classic, Compendium of Materia Medic, and described as possessing features that invigorate the spleen, alleviate dampness, provide diarrhea relief, and other effects. The DLL-dried white mature seeds (DS) and dried flower (DF), which hold significant medicinal value in China, were used in clinical prescriptions to prevent and treat UC. DS and DF have appeared in different editions of the Pharmacopoeia of the People's Republic of China from 1977 to 2020. However, their chemical composition, pharmacological effects, and mechanism of treating UC are unclear. Aim of the Study: This study aimed to characterize the chemical composition of different parts of DLL (seeds and flowers), further explore their pharmacological effects, and elaborate its underlying mechanism of treating UC. Methods: The chemical composition of DS and DF crude polysaccharides (DSP and DFP) and ethanolic extracts (DSE and DFE) were characterized by high-performance anion-exchange chromatography (HPAEC), ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS), and gas chromatography-mass spectrometry (GC-MS). Then, based on the acute UC mice model, the pharmacodynamic effects were investigated by Western blotting, ELISA, and other methods. Finally, the 16S rRNA gene sequencing and metabonomic analysis were used to explore the regulatory effects of DS and DF on intestinal microbiota and host metabolism. Results: DSE and DFE inhibited the oxidative stress response, reducing proinflammatory factor production and maintaining intestinal barrier integrity in UC mice. The 16S rRNA gene sequencing and metabonomic analysis revealed that DS and DF treated UC by regulating the intestinal microbiota structure and reversing the abnormal metabolism of the host. Conclusion: This study suggested that different parts of DLL (flowers and seeds) may be potential medicines for treating UC, which exert their therapeutic effects through various active ingredients and might contribute significantly to reducing the economic pressures and challenges of UC treatment worldwide. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Dolichos lablab L.; Gut bacteria; Metabolomics; Ulcerative colitis |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 9042-14-2 (Dextran Sulfate) |
| تواريخ الأحداث: | Date Created: 20231222 Date Completed: 20240115 Latest Revision: 20240115 |
| رمز التحديث: | 20240115 |
| DOI: | 10.1016/j.jep.2023.117629 |
| PMID: | 38135234 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2023.117629 |