Physiological Influence of Fe and Cu Availability on Nitrogen Isotope Fractionation during Ammonia Oxidation.

التفاصيل البيبلوغرافية
العنوان:	Physiological Influence of Fe and Cu Availability on Nitrogen Isotope Fractionation during Ammonia Oxidation.
المؤلفون:	Martocello DE; Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, United States.; Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, United States., Wankel SD; Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, United States.
المصدر:	Environmental science & technology [Environ Sci Technol] 2024 Jan 09; Vol. 58 (1), pp. 421-431. Date of Electronic Publication: 2023 Dec 26.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 0213155 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5851 (Electronic) Linking ISSN: 0013936X NLM ISO Abbreviation: Environ Sci Technol Subsets: MEDLINE
أسماء مطبوعة:	Publication: Washington DC : American Chemical Society Original Publication: Easton, Pa. : American Chemical Society, c1967-
مواضيع طبية MeSH:	Ammonia/metabolism , Ecosystem, Nitrogen Isotopes/metabolism ; Archaea/metabolism ; Nitrification ; Nitrogen/metabolism ; Oxidation-Reduction ; Soil Microbiology ; Phylogeny
مستخلص:	Microbially mediated cycling processes play central roles in regulating the speciation and availability of nitrogen, a vital nutrient with wide implications for agriculture, water quality, wastewater treatment, ecosystem health, and climate change. Ammonia oxidation, the first and rate-limiting step of nitrification, is carried out by bacteria (AOB) and archaea (AOA) that require the trace metal micronutrients copper (Cu) and iron (Fe) for growth and metabolic catalysis. While stable isotope analyses for constraining nitrogen cycling are commonly used, it is unclear whether metal availability may modulate expression of stable isotope fractionation during ammonia oxidation, by varying growth or through regulation of metabolic metalloenzymes. We present the first study examining the influence of Fe and Cu availability on the kinetic nitrogen isotope effect in ammonia oxidation ( ¹⁵ ε AO ). We report a general independence of ¹⁵ ε AO from the growth rate in AOB, except at a low temperature (10 °C). With AOA Nitrosopumilus maritimus SCM1, however, ¹⁵ ε AO decreases nonlinearly at lower oxidation rates. We examine assumptions involved in the interpretation of ¹⁵ ε AO values and suggest these dynamics may arise from physiological constraints that push the system toward isotopic equilibrium. These results suggest important links between isotope fractionation and environmental constraints on physiology in these key N cycling microorganisms.
فهرسة مساهمة:	Keywords: ammonia oxidation; enzyme reversibility; metal limitation; nitrification; stable isotopes
المشرفين على المادة:	0 (Nitrogen Isotopes) 7664-41-7 (Ammonia) N762921K75 (Nitrogen)
تواريخ الأحداث:	Date Created: 20231226 Date Completed: 20240110 Latest Revision: 20240110
رمز التحديث:	20240110
DOI:	10.1021/acs.est.3c05964
PMID:	38147309
قاعدة البيانات:	MEDLINE

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الوصف
تدمد:	1520-5851
DOI:	10.1021/acs.est.3c05964