دورية أكاديمية
أعرض في EDS

Secondary Data Analysis of Inflammation-Related mRNAs in Conjunctival Impression Cytology Samples of Aniridia Patients.

التفاصيل البيبلوغرافية
العنوان: Secondary Data Analysis of Inflammation-Related mRNAs in Conjunctival Impression Cytology Samples of Aniridia Patients.
المؤلفون: Stachon T; Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Homburg, Saarland, Germany; and., Latta L; Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Homburg, Saarland, Germany; and., Fries FN; Department of Ophthalmology, Saarland University Medical Center, Homburg, Saarland, Germany., Seitz B; Department of Ophthalmology, Saarland University Medical Center, Homburg, Saarland, Germany., Szentmáry N; Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Homburg, Saarland, Germany; and.
المصدر: Cornea [Cornea] 2024 May 01; Vol. 43 (5), pp. 627-634. Date of Electronic Publication: 2023 Dec 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 8216186 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1536-4798 (Electronic) Linking ISSN: 02773740 NLM ISO Abbreviation: Cornea Subsets: MEDLINE
أسماء مطبوعة: Publication: Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: New York, N.Y. : Masson Pub. USA, c1982-
مواضيع طبية MeSH: Corneal Diseases*/complications , Aniridia*/genetics , Aniridia*/complications , Corneal Neovascularization*/complications, Humans ; RNA, Messenger/genetics ; Secondary Data Analysis ; Cytology ; Inflammation/genetics ; Vision Disorders ; Cytokines/genetics
مستخلص: Purpose: Aniridia is a rare corneal disease that is often associated with aniridia-associated keratopathy (AAK). In AAK, the conjunctival tissue crosses the limbal border, forming a corneal pannus that extends into the corneal center. With increasing AAK severity, corneal pannus formation, vascularization, and ocular surface inflammation increase. The purpose of this study was to investigate inflammation-related mRNA expression in conjunctival epithelial cells in AAK and its relationship with AAK severity.
Methods: Using impression cytology, bulbar conjunctival cells were sampled from 20 subjects with congenital aniridia and 20 age-matched and sex-matched healthy control subjects. RNA was extracted, and mRNA analyses were performed using microarray, which was evaluated for inflammatory markers.
Results: In the analyzed aniridia subjects, 70 deregulated mRNAs encoding proinflammatory or antiinflammatory cytokines or factors associated with chronic inflammation, including increased IL-1, IL-8, and MIP3A/CCL20 mRNA. The most downregulated mRNA was TIMP3, and the most upregulated mRNA was Protein c-Fos.Of the 70 mRNAs, 14 inflammation-related genes were altered only in the mild AAK forms, whereas only 2 mRNAs were altered only in the severe AAK forms (TLR4 and PPARG).
Conclusions: The expression of numerous proinflammatory and antiinflammatory cytokines is deregulated at the ocular surface of aniridia subjects with mild AAK. Thus, early antiinflammatory treatment may prevent or slow down corneal scarring and pannus formation in aniridia subjects.
Competing Interests: The authors have no funding or conflicts of interest to disclose.
(Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.)
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المشرفين على المادة: 0 (RNA, Messenger)
0 (Cytokines)
تواريخ الأحداث: Date Created: 20231226 Date Completed: 20240403 Latest Revision: 20240403
رمز التحديث: 20240403
DOI: 10.1097/ICO.0000000000003454
PMID: 38147570
قاعدة البيانات: MEDLINE
الوصف
تدمد:1536-4798
DOI:10.1097/ICO.0000000000003454