دورية أكاديمية
Enteric glial cell network function is required for epithelial barrier restitution following intestinal ischemic injury in the early postnatal period.
| العنوان: | Enteric glial cell network function is required for epithelial barrier restitution following intestinal ischemic injury in the early postnatal period. |
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| المؤلفون: | Ziegler AL; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Caldwell ML; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Craig SE; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Hellstrom EA; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Sheridan AE; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Touvron MS; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Pridgen TA; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Magness ST; Joint Department of Biomedical Engineering, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, United States., Odle J; Department of Animal Science, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, North Carolina, United States., Van Landeghem L; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States., Blikslager AT; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States. |
| المصدر: | American journal of physiology. Gastrointestinal and liver physiology [Am J Physiol Gastrointest Liver Physiol] 2024 Mar 01; Vol. 326 (3), pp. G228-G246. Date of Electronic Publication: 2023 Dec 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901227 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1547 (Electronic) Linking ISSN: 01931857 NLM ISO Abbreviation: Am J Physiol Gastrointest Liver Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society |
| مواضيع طبية MeSH: | Neuroglia*/physiology , Intestine, Small*, Humans ; Animals ; Infant, Newborn ; Swine ; Intestines ; Intestinal Mucosa ; Jejunum ; Ischemia |
| مستخلص: | Ischemic damage to the intestinal epithelial barrier, such as in necrotizing enterocolitis or small intestinal volvulus, is associated with higher mortality rates in younger patients. We have recently reported a powerful pig model to investigate these age-dependent outcomes in which mucosal barrier restitution is strikingly absent in neonates but can be rescued by direct application of homogenized mucosa from older, juvenile pigs by a yet-undefined mechanism. Within the mucosa, a postnatally developing network of enteric glial cells (EGCs) is gaining recognition as a key regulator of the mucosal barrier. Therefore, we hypothesized that the developing EGC network may play an important role in coordinating intestinal barrier repair in neonates. Neonatal and juvenile jejunal mucosa recovering from surgically induced intestinal ischemia was visualized by scanning electron microscopy and the transcriptomic phenotypes were assessed by bulk RNA sequencing. EGC network density and glial activity were examined by Gene Set Enrichment Analysis, three-dimensional (3-D) volume imaging, and Western blot and its function in regulating epithelial restitution was assessed ex vivo in Ussing chamber using the glia-specific inhibitor fluoroacetate (FA), and in vitro by coculture assay. Here we refine and elaborate our translational model, confirming a neonatal phenotype characterized by a complete lack of coordinated reparative signaling in the mucosal microenvironment. Furthermore, we report important evidence that the subepithelial EGC network changes significantly over the early postnatal period and demonstrate that the proximity of a specific functional population of EGC to wounded intestinal epithelium contributes to intestinal barrier restitution following ischemic injury. NEW & NOTEWORTHY This study refines a powerful translational pig model, defining an age-dependent relationship between enteric glia and the intestinal epithelium during intestinal ischemic injury and confirming an important role for enteric glial cell (EGC) activity in driving mucosal barrier restitution. This study suggests that targeting the enteric glial network could lead to novel interventions to improve recovery from intestinal injury in neonatal patients. |
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| معلومات مُعتمدة: | P30 DK034987 United States DK NIDDK NIH HHS; R01 HD095876 United States HD NICHD NIH HHS; T32 DK007737 United States DK NIDDK NIH HHS; L40 OD028437 United States OD NIH HHS; K01 OD028207 United States OD NIH HHS; L40 OD032118 United States OD NIH HHS |
| فهرسة مساهمة: | Keywords: enteric glia; epithelial restitution; intestinal barrier; intestinal ischemia; restitution |
| تواريخ الأحداث: | Date Created: 20231226 Date Completed: 20240214 Latest Revision: 20240629 |
| رمز التحديث: | 20240629 |
| مُعرف محوري في PubMed: | PMC11211042 |
| DOI: | 10.1152/ajpgi.00216.2022 |
| PMID: | 38147796 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1547 |
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| DOI: | 10.1152/ajpgi.00216.2022 |