التفاصيل البيبلوغرافية
| العنوان: |
Ultrastructural study: in vitro and in vivo differentiation of mice spermatogonial stem cells. |
| المؤلفون: |
Bashiri Z; Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.; Omid Fertility & Infertility Clinic, Hamedan, Iran., Movahedin M; Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran., Pirhajati V; Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran., Asgari H; Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran., Koruji M; Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. |
| المصدر: |
Zygote (Cambridge, England) [Zygote] 2024 Feb; Vol. 32 (1), pp. 87-95. Date of Electronic Publication: 2023 Dec 27. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Cambridge University Press Country of Publication: England NLM ID: 9309124 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8730 (Electronic) Linking ISSN: 09671994 NLM ISO Abbreviation: Zygote Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Cambridge ; New York, NY : Cambridge University Press, c1993- |
| مواضيع طبية MeSH: |
Spermatogonia* , Seminiferous Tubules*, Male ; Mice ; Animals ; Testis ; Spermatozoa ; Spermatogenesis ; Stem Cells ; Mammals |
| مستخلص: |
Mouse testicular tissue is composed of seminiferous tubules and interstitial tissue. Mammalian spermatogenesis is divided into three stages: spermatocytogenesis (mitotic divisions) in which spermatogonial stem cells (SSCs) turn into spermatocytes, followed by two consecutive meiotic divisions in which spermatocytes form spermatids. Spermatids differentiate into spermatozoa during spermiogenesis. Various factors affect the process of spermatogenesis and the organization of cells in the testis. Any disorder in different stages of spermatogenesis will have negative effects on male fertility. The aim of the current study was to compare the in vitro and in vivo spermatogenesis processes before and after transplantation to azoospermic mice using ultrastructural techniques. In this study, mice were irradiated with single doses of 14 Gy 60 Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitro culture are similar to those of in vivo spermatogenesis, indicating that spermatogenic cell nature is unaltered in vitro . |
| فهرسة مساهمة: |
Keywords: In vitro culture; Irradiation; Spermatogonia stem cells; Transplantation; Ultrastructure |
| تواريخ الأحداث: |
Date Created: 20231227 Date Completed: 20240116 Latest Revision: 20240116 |
| رمز التحديث: |
20240116 |
| DOI: |
10.1017/S096719942300062X |
| PMID: |
38149356 |
| قاعدة البيانات: |
MEDLINE |
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