دورية أكاديمية
Chemical and microbial diversity of a tropical intertidal ascidian holobiont.
| العنوان: | Chemical and microbial diversity of a tropical intertidal ascidian holobiont. |
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| المؤلفون: | Bauermeister A; Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, 05508-000, Brazil; Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil., Furtado LC; Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, 05508-000, Brazil., Ferreira EG; Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza, CE, 60451-970, Brazil., Moreira EA; Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil., Jimenez PC; Instituto do Mar, Universidade Federal de São Paulo, Santos, SP, 11.070-100, Brazil., Lopes NP; Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil., Araújo WL; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, 05508-900, Brazil., Olchanheski LR; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, 05508-900, Brazil., Monteiro da Cruz Lotufo T; Instituto Oceanográfico, Universidade de São Paulo, São Paulo, SP, 05508-120, Brazil., Costa-Lotufo LV; Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, 05508-000, Brazil. Electronic address: costalotufo@usp.br. |
| المصدر: | Marine environmental research [Mar Environ Res] 2024 Feb; Vol. 194, pp. 106303. Date of Electronic Publication: 2023 Dec 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 9882895 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0291 (Electronic) Linking ISSN: 01411136 NLM ISO Abbreviation: Mar Environ Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Apr. <2000- >: Kidlington, Oxford : Elsevier Original Publication: London : Applied Science Publishers, [c1978- |
| مواضيع طبية MeSH: | Urochordata*, Animals ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Tandem Mass Spectrometry ; Bacteria/genetics |
| مستخلص: | The tropical ascidian Eudistoma vannamei, endemic to the northeastern coast of Brazil, is considered a prolific source of secondary metabolites and hosts Actinomycetota that produce bioactive compounds. Herein, we used an omics approach to study the ascidian as a holobiont, including the microbial diversity through 16S rRNA gene sequencing and metabolite production using mass spectrometry-based metabolomics. Gene sequencing analysis revealed all samples of E. vannamei shared about 50% of the observed ASVs, and Pseudomonadota (50.7%), Planctomycetota (9.58%), Actinomycetota (10.34%), Bacteroidota (12.05%) were the most abundant bacterial phyla. Analysis of tandem mass spectrometry (MS/MS) data allowed annotation of compounds, including phospholipids, amino acids, and pyrimidine alkaloids, such as staurosporine, a member of a well-known chemical class recognized as a microbial metabolite. Isolated bacteria, mainly belonging to Streptomyces and Micromonospora genera, were cultivated and extracted with ethyl acetate. MS/MS analysis of bacterial extracts allowed annotation of compounds not detected in the ascidian tissue, including marineosin and dihydroergotamine, yielding about 30% overlapped ions between host and isolated bacteria. This study reveals E. vannamei as a rich source of microbial and chemical diversity and, furthermore, highlights the importance of omic tools for a comprehensive investigation of holobiont systems. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 16S rRNA diversity; Marine natural products; Mass spectrometry; Metabolomics; Microbiome; Tunicate |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20231227 Date Completed: 20240122 Latest Revision: 20240122 |
| رمز التحديث: | 20240122 |
| DOI: | 10.1016/j.marenvres.2023.106303 |
| PMID: | 38150785 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0291 |
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| DOI: | 10.1016/j.marenvres.2023.106303 |