دورية أكاديمية
أعرض في EDS

Differential Gene Regulation of the Human Blastocyst Trophectoderm and Inner Cell Mass by Progesterone.

التفاصيل البيبلوغرافية
العنوان: Differential Gene Regulation of the Human Blastocyst Trophectoderm and Inner Cell Mass by Progesterone.
المؤلفون: Snow KS; Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynecology, Duke University, 5601 Arringdon Park Dr Suite 210, Morrisville, NC, 27560, USA., Raburn DJ; Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynecology, Duke University, 5601 Arringdon Park Dr Suite 210, Morrisville, NC, 27560, USA., Price TM; Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynecology, Duke University, 5601 Arringdon Park Dr Suite 210, Morrisville, NC, 27560, USA. price067@mc.duke.edu.
المصدر: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 May; Vol. 31 (5), pp. 1363-1372. Date of Electronic Publication: 2023 Dec 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 101291249 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1933-7205 (Electronic) Linking ISSN: 19337191 NLM ISO Abbreviation: Reprod Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [New York] : Springer
Original Publication: Thousand Oaks, Calif. : Sage
مواضيع طبية MeSH: Progesterone*/pharmacology , Blastocyst*/metabolism , Blastocyst*/drug effects , Gene Expression Regulation, Developmental* , Blastocyst Inner Cell Mass*/metabolism, Humans ; Receptors, Progesterone/metabolism ; Receptors, Progesterone/genetics ; Female ; Embryonic Development/drug effects ; Embryonic Development/physiology ; Human Embryonic Stem Cells/metabolism ; Human Embryonic Stem Cells/drug effects
مستخلص: Knowledge of action of progesterone (P4) on the human preimplantation embryo is lacking. The objective of this study was to determine expression of a mitochondrial P4 receptor (PR-M) in the trophectoderm (TE) and the inner cell mass (ICM) of the human blastocyst and to determine P4-induced gene expression during growth from the cleavage to the blastocyst stage. Previously cryopreserved cleavage stage embryos were treated with P4 (10 -6 M) or vehicle until blastocyst development. Cells from the TE and the ICM of dissected euploid embryos underwent RNA-seq analysis, while other embryos were used for analysis of nuclear PR (nPR) and PR-M expression.PR-M expression was confirmed in the TE, the ICM, and a human embryonic stem cell line (HESC). Conversely, nPR expression was absent in the TE and the ICM with low expression in the HESC line. RNA-seq analysis revealed P4 effects greater in the TE with 183 significant pathway changes compared to 27 in the ICM. The TE response included significant upregulation of genes associated with DNA replication, cell cycle phase transition and others, exemplified by a 7.6-fold increase in the cell proliferation gene, F-Box Associated Domain Containing. The majority of ICM pathways were downregulated including chromosome separation, centromere complex assembly and chromatin remodeling at centromere. This study confirms that human blastocysts express PR-M in both the TE and the ICM, but lack expression of nPR. P4-induced gene regulation differs greatly in the two cell fractions with the predominant effect of cell proliferation in the TE and not the ICM.
(© 2023. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
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فهرسة مساهمة: Keywords: Blastocyst gene expression; Embryo culture media; P4 supplementation; RNA-seq
المشرفين على المادة: 4G7DS2Q64Y (Progesterone)
0 (Receptors, Progesterone)
تواريخ الأحداث: Date Created: 20231227 Date Completed: 20240513 Latest Revision: 20240925
رمز التحديث: 20240925
DOI: 10.1007/s43032-023-01429-z
PMID: 38151652
قاعدة البيانات: MEDLINE
الوصف
تدمد:1933-7205
DOI:10.1007/s43032-023-01429-z