دورية أكاديمية
[Association of polymorphic variants of hemostatic system genes with the course of COVID-19].
| العنوان: | [Association of polymorphic variants of hemostatic system genes with the course of COVID-19]. |
|---|---|
| المؤلفون: | Nikolaeva LI; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Stuchinskaya MD; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Dedova AV; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Nadezhda SG; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Khlopova IN; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Kruzhkova IS; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia.; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health., Merkulova LN; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Kisteneva LB; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Kolobukhina LV; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia.; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health., Mukasheva EA; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Krasnoslobodtsev KG; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Trushakova SV; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Krepkaya AS; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Kuprianov VV; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Nikitenko NA; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Khadorich EA; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Burmistrov EM; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia., Tyurin IN; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health., Antipyat NA; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health., Burtseva EI; N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia. |
| المصدر: | Voprosy virusologii [Vopr Virusol] 2023 Nov 07; Vol. 68 (5), pp. 445-453. Date of Electronic Publication: 2023 Nov 07. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora Country of Publication: Russia (Federation) NLM ID: 0417337 Publication Model: Electronic Cited Medium: Internet ISSN: 2411-2097 (Electronic) Linking ISSN: 05074088 NLM ISO Abbreviation: Vopr Virusol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Moskva : FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora Original Publication: Moskva : Ministerstvo zdravookhranenii︠a︡ SSSR |
| مواضيع طبية MeSH: | Hemostatics* , COVID-19*/epidemiology , COVID-19*/genetics, Humans ; Asymptomatic Infections ; Genotype ; Hemostasis/genetics ; DNA ; Plasminogen Activator Inhibitor 1/genetics |
| مستخلص: | Introduction: COVID-19 is characterized by a varied clinical course. The aim of the work was to identify associations of SNPs of hemostatic system genes with COVID-19. Materials and Methods: DNA was isolated from patients (n=117) and healthy participants (n=104). All infected patients were divided into 3 groups, depending on disease severity assessment, which was appreciated by NEWS2. Another group consisted of participants, who had asymptomatic infection in the past. Determination of SNPs of the genes FGB (-455 G/A), FII (20210 G/A), FV (1691 G/A), FVII (10976 G/A), FXIIIA1 (103 G/T), ITGA2 (807 C/T), ITGB3 (1565 T/C), SERPINE1 (-675 5G/4G) were performed by PCR using the "Genetics of Hemostasis" kit ("DNA-Technology", Russia). Results: In analyzed SNPs, no significant differences were detected between the group of infected patients and healthy participants. But significant association was revealed in gene SERPINE1 (-675 5G/4G), when patient groups, differing in the disease severity, were analyzed relative to the group of participants with asymptomatic infection (p=0.0381; p=0 .0066; p=0.0009). It was found, that as COVID-19 severity scores increased, the proportion of 5G allele of gene SERPINE1 decreased, and the proportion of the 4G allele increased (p=0.005; p=0.009; p=0.0005). Similar processes were observed for genotypes 5G/5G and 4G/4G. Discussion: The gene SERPINE1 (-675 5G/4G) is associated with the severity of COVID-19. Conclusion: For the first time, it was discovered that 5G/5G genotype of gene SERPINE1 (-675 5G/4G) can be a marker of a milder course of COVID-19, and the 4G/4G genotype as a more severe one. |
| فهرسة مساهمة: | Keywords: COVID-19; association with clinical course; hemostatic gene polymorphism Local Abstract: [Publisher, Russian] Введение. COVID-19 характеризуется разнообразным течением: от бессимптомного до тяжелого с летальным исходом. Комплекс неблагоприятных факторов инфицированного организма во многом определяет течение и исход болезни. Цель работы – выявить возможные ассоциации полиморфных генов системы гемостаза с течением COVID-19. Материалы и методы. ДНК выделяли из крови инфицированных пациентов (n=117) и участников группы сравнения (n=104). Все инфицированные были поделены на 3 группы в зависимости от тяжести состояния, которую оценивали по NEWS2. Еще одну группу составили участники, перенесшие инфекцию бессимптомно. Определение однонуклеотидного полиморфизма (ОНП) генов FGB (-455 G/A), FII (20210 G/A), FV (1691 G/A), FVII (10976 G/A), FXIIIA1 (103 G/T), ITGA2 (807 C/T), ITGB3 (1565 T/C), SERPINE1 (-675 5G/4G) проводили методом ПЦР, используя набор «Генетика гемостаза» («ДНК-Технология», Россия). Результаты. Достоверных различий в аллельных вариантах анализируемых генов между группой всех инфицированных и здоровыми участниками не выявлено. Но анализ групп пациентов, различающихся по тяжести COVID-19, относительно группы участников, бессимптомно перенесших инфекцию, показал, что в гене SERPINE1 (-675 5G/4G) обнаружена достоверная ассоциация ОНП с течением инфекции (р=0,0381; р=0,0066; р=0,0009). Установлено, что по мере увеличения баллов тяжести COVID-19 доля аллеля 5G гена SERPINE1 (-675 5G/4G) снижалась, а доля аллеля 4G увеличивалась (р=0,005; р=0,009; р=0,0005). Аналогичные процессы наблюдались для генотипов 5G/5G и 4G/4G. Обсуждение. Анализ вклада ОНП генов системы гемостаза показал, что фибринолитическое звено ассоциировано с тяжестью COVID-19. Полиморфизм гена этого звена SERPINE1 (-675 5G/4G) имел достоверную связь с течением COVID-19. Заключение. Впервые обнаружено, что ОНП гена SERPINE1 (-675 5G/4G) ассоциирован с течением COVID-19. Генотип 5G/5G этого гена может рассматриваться как маркер более легкого течения инфекции, генотип 4G/4G – более тяжелого. |
| المشرفين على المادة: | 0 (Hemostatics) 9007-49-2 (DNA) 0 (Plasminogen Activator Inhibitor 1) |
| تواريخ الأحداث: | Date Created: 20231229 Date Completed: 20240101 Latest Revision: 20240101 |
| رمز التحديث: | 20240101 |
| DOI: | 10.36233/0507-4088-197 |
| PMID: | 38156578 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2411-2097 |
|---|---|
| DOI: | 10.36233/0507-4088-197 |