دورية أكاديمية
Monitoring the Photorhabdus spp. bacterial load in Heterorhabditis bacteriophora dauer juveniles over different storage times and temperatures: A molecular approach.
| العنوان: | Monitoring the Photorhabdus spp. bacterial load in Heterorhabditis bacteriophora dauer juveniles over different storage times and temperatures: A molecular approach. |
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| المؤلفون: | Ogaya C; Faculty of Agricultural and Nutritional Sciences, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 4, 24118 Kiel, Germany; e-nema GmbH, Klausdorfer Str. 28-36, 24223 Schwentinental, Germany., Huong N; Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium., Touceda-González M; e-nema GmbH, Klausdorfer Str. 28-36, 24223 Schwentinental, Germany., Barg M; e-nema GmbH, Klausdorfer Str. 28-36, 24223 Schwentinental, Germany., Dörfler V; e-nema GmbH, Klausdorfer Str. 28-36, 24223 Schwentinental, Germany., Ehlers RU; Faculty of Agricultural and Nutritional Sciences, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 4, 24118 Kiel, Germany; Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium., Molina C; e-nema GmbH, Klausdorfer Str. 28-36, 24223 Schwentinental, Germany. Electronic address: c.molina@e-nema.de. |
| المصدر: | Journal of invertebrate pathology [J Invertebr Pathol] 2024 Mar; Vol. 203, pp. 108048. Date of Electronic Publication: 2023 Dec 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0014067 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0805 (Electronic) Linking ISSN: 00222011 NLM ISO Abbreviation: J Invertebr Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Photorhabdus*/genetics, Animals ; Temperature ; DNA, Bacterial/genetics ; Bacterial Load ; Genome ; Symbiosis |
| مستخلص: | Biological control products based on the entomopathogenic nematode Heterorhabditis bacteriophora can vary in virulence (quality). The influence of their symbiotic bacteria Photorhabdus spp. inside the infective dauer juvenile (DJ) on DJ quality has not received much attention in the past. The presence of the bacteria in the DJ is crucial for its biocontrol potential. This investigation provides a method to quantify the bacterial load inside the DJ based on a qPCR technique. Information from the genome of Photorhabdus laumondii strain DE2 was used to identify single copy genes with no homology to any other bacterial accessions. One gene (hereby named CG2) was selected for primers design and for further qPCR experiments. Cross-amplification tests with P. thracensis and P. kayaii, also symbionts of H. bacteriophora, were positive, whereas no amplicons were produced for P. temperata or Xenorhabdus nematophila. We tested our qPCR system in DJ populations carrying defined proportions of bacteria-free (axenic) vs bacteria-carrying nematodes. With an increasing proportion of axenic DJ in a population, virulence declined, and the virulence was proportional to the amount of bacterial DNA detected in the population by qPCR. Along liquid storage over long time, virulence also decreased, and this factor correlated with the reduction of bacterial DNA on the respective DJ population. We observed that stored DJ kept virulent up to 90 days and thereafter the virulence as well as the amount of bacterial DNA drastically decreased. Storage temperature also influenced the bacterial survival. Inside formulated DJ, the loss of bacterial DNA on the DJ population was accelerated under storage temperatures below 7.5 °C, suggesting that reproduction of the bacterial cells takes place when growth temperature is favorable. The role of bacterial survival inside stored DJ can now be adequately addressed using this molecular quality-control technique. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Absolute quantification; Bacteria load; Entomopathogenic nematodes; Relative quantification; Single copy gene; Virulence; qPCR |
| المشرفين على المادة: | 0 (DNA, Bacterial) |
| تواريخ الأحداث: | Date Created: 20231230 Date Completed: 20240313 Latest Revision: 20240313 |
| رمز التحديث: | 20240313 |
| DOI: | 10.1016/j.jip.2023.108048 |
| PMID: | 38159796 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0805 |
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| DOI: | 10.1016/j.jip.2023.108048 |