دورية أكاديمية
The YBX3 RNA-binding protein posttranscriptionally controls SLC1A5 mRNA in proliferating and differentiating skeletal muscle cells.
| العنوان: | The YBX3 RNA-binding protein posttranscriptionally controls SLC1A5 mRNA in proliferating and differentiating skeletal muscle cells. |
|---|---|
| المؤلفون: | Awad S; Biology Department, Haverford College, Haverford, Pennsylvania, USA., Skipper W; Biology Department, Haverford College, Haverford, Pennsylvania, USA., Vostrejs W; Biology Department, Haverford College, Haverford, Pennsylvania, USA., Ozorowski K; Biology Department, Haverford College, Haverford, Pennsylvania, USA., Min K; Biology Department, Haverford College, Haverford, Pennsylvania, USA., Pfuhler L; Biology Department, Haverford College, Haverford, Pennsylvania, USA., Mehta D; Biology Department, Haverford College, Haverford, Pennsylvania, USA., Cooke A; Biology Department, Haverford College, Haverford, Pennsylvania, USA. Electronic address: acooke1@haverford.edu. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2024 Feb; Vol. 300 (2), pp. 105602. Date of Electronic Publication: 2023 Dec 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Amino Acid Transport System ASC*/metabolism , Muscle Fibers, Skeletal*/metabolism , RNA-Binding Proteins*/genetics , RNA-Binding Proteins*/metabolism, Animals ; Humans ; Mice ; Amino Acids/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Gene Expression Regulation/genetics ; NIH 3T3 Cells ; HCT116 Cells ; Cell Proliferation/genetics ; Cell Differentiation/genetics |
| مستخلص: | In humans, skeletal muscles comprise nearly 40% of total body mass, which is maintained throughout adulthood by a balance of muscle protein synthesis and breakdown. Cellular amino acid (AA) levels are critical for these processes, and mammalian cells contain transporter proteins that import AAs to maintain homeostasis. Until recently, the control of transporter regulation has largely been studied at the transcriptional and posttranslational levels. However, here, we report that the RNA-binding protein YBX3 is critical to sustain intracellular AAs in mouse skeletal muscle cells, which aligns with our recent findings in human cells. We find that YBX3 directly binds the solute carrier (SLC)1A5 AA transporter messenger (m)RNA to posttranscriptionally control SLC1A5 expression during skeletal muscle cell differentiation. YBX3 regulation of SLC1A5 requires the 3' UTR. Additionally, intracellular AAs transported by SLC1A5, either directly or indirectly through coupling to other transporters, are specifically reduced when YBX3 is depleted. Further, we find that reduction of the YBX3 protein reduces proliferation and impairs differentiation in skeletal muscle cells, and that YBX3 and SLC1A5 protein expression increase substantially during skeletal muscle differentiation, independently of their respective mRNA levels. Taken together, our findings suggest that YBX3 regulates AA transport in skeletal muscle cells, and that its expression is critical to maintain skeletal muscle cell proliferation and differentiation. Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | R15 GM146205 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: 3′ untranslated region (UTR); C2C12 myoblasts; RNA-binding protein; SLC1A5; SLC3A2; SLC7A5; YBX3; amino acid transport |
| المشرفين على المادة: | 0 (Amino Acid Transport System ASC) 0 (Amino Acids) 0 (RNA, Messenger) 0 (RNA-Binding Proteins) 0 (SLC1A5 protein, human) 0 (Csda protein, mouse) 0 (Slc1a5 protein, mouse) 0 (YBX3 protein, human) |
| تواريخ الأحداث: | Date Created: 20231230 Date Completed: 20240226 Latest Revision: 20240227 |
| رمز التحديث: | 20240227 |
| مُعرف محوري في PubMed: | PMC10837625 |
| DOI: | 10.1016/j.jbc.2023.105602 |
| PMID: | 38159852 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1083-351X |
|---|---|
| DOI: | 10.1016/j.jbc.2023.105602 |