دورية أكاديمية
Histochemical and morphological evaluation of a glyoxal acid-free fixative.
| العنوان: | Histochemical and morphological evaluation of a glyoxal acid-free fixative. |
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| المؤلفون: | Peeler C; Department of Diagnostic and Health Sciences, College of Health Professions, University of Tennessee Health Science Center, Memphis, TN, USA., Pitzer CR; Department of Physiology, College of Graduate Health Sciences, University of Tennessee Health Science Center, Memphis, TN, USA., Paez HG; Department of Physiology, College of Graduate Health Sciences, University of Tennessee Health Science Center, Memphis, TN, USA., Criswell S; Department of Diagnostic and Health Sciences, College of Health Professions, University of Tennessee Health Science Center, Memphis, TN, USA. |
| المصدر: | Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 2024 Nov; Vol. 99 (1), pp. 49-58. Date of Electronic Publication: 2024 Jan 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9107378 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-7760 (Electronic) Linking ISSN: 10520295 NLM ISO Abbreviation: Biotech Histochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Abingdon, Oxford : Taylor & Francis Original Publication: Baltimore; MD : Williams & Wilkins, c1991- |
| مواضيع طبية MeSH: | Glyoxal*/chemistry , Formaldehyde*/chemistry, Fixatives/chemistry ; Tissue Fixation ; Histocytochemistry |
| مستخلص: | The application of most chemical fixatives, such as formalin, in the anatomic pathology laboratory requires safety training and hazardous chemical monitoring due to the toxicity and health risks associated with their use. Consequently, the use of formalin has been banned in most applications in Europe; the primary exception is its use in the histology laboratory in lieu of a suitable and safer alternative. Glyoxal based solutions, several of which are available commercially, are the most promising alternative fixatives, because they are based on a mechanism of fixation similar to that of formalin. Unlike formalin, however, glyoxal based solutions do not dissociate from water and therefore do not require ventilation measures such as a fume hood. A primary barrier to the adoption of commercially available glyoxal based solutions is their low pH, which can produce undesirable morphological and antigenic tissue alterations; however, a recently available neutral pH glyoxal product (glyoxal acid free) (GAF) has been developed to mitigate the challenges of low pH. We compared the morphology and histochemistry among tissues fixed in 10% neutral buffered formalin, a commercially available acidic glyoxal product (Prefer), and GAF. Tissues fixed in formalin and Prefer exhibited similar morphology and staining properties; tissues fixed with 2% GAF exhibited deleterious effects. |
| فهرسة مساهمة: | Keywords: Bacteria; fixation; formalin; fungi; glyoxal acid; glyoxal acid free; histology; human; mice; tissue storage |
| المشرفين على المادة: | 0 (Fixatives) 50NP6JJ975 (Glyoxal) 1HG84L3525 (Formaldehyde) |
| تواريخ الأحداث: | Date Created: 20240102 Date Completed: 20240110 Latest Revision: 20240110 |
| رمز التحديث: | 20240110 |
| DOI: | 10.1080/10520295.2023.2300797 |
| PMID: | 38164087 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1473-7760 |
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| DOI: | 10.1080/10520295.2023.2300797 |