دورية أكاديمية
أعرض في EDS

Optimization of media components for enhanced carotenoid production by Paracoccus marcusii RSPO1 and assessment of their cytotoxicity against A549 and vero cells.

التفاصيل البيبلوغرافية
العنوان: Optimization of media components for enhanced carotenoid production by Paracoccus marcusii RSPO1 and assessment of their cytotoxicity against A549 and vero cells.
المؤلفون: Naik R; Department of Microbiology, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Science (ARIBAS), CVM University, Anand, Gujarat, India., Gupte S; Department of Microbiology, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Science (ARIBAS), CVM University, Anand, Gujarat, India.
المصدر: Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2024 Jul; Vol. 54 (6), pp. 764-778. Date of Electronic Publication: 2024 Jan 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9607037 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2297 (Electronic) Linking ISSN: 10826068 NLM ISO Abbreviation: Prep Biochem Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005-> : Abingdon, Oxford : Taylor & Francis
Original Publication: New York, NY : Marcel Dekker, c1996-
مواضيع طبية MeSH: Carotenoids*/pharmacology , Carotenoids*/metabolism , Paracoccus*/metabolism, Chlorocebus aethiops ; Animals ; Humans ; A549 Cells ; Vero Cells ; Culture Media/chemistry ; Reactive Oxygen Species/metabolism
مستخلص: In this study, we tried to explore the influence of various tricarboxylic acid (TCA) cycle intermediates on carotenoid production and with a focus on enhancing pigment biosynthesis, we conducted two statistical analysis. In case of TCA intermediates influence on pigment production by Paracoccus marcusii RSPO1; fumaric acid, and malic acid were observed as potent enhancers of pigment biosynthesis. Further, to optimize key media components for enhanced carotenoid production, the Plackett-Burman design was employed encompassing carbon, nitrogen sources, TCA cycle intermediates, and metal salts. The selected factors after Plackett Burman were fine-tuned through Response Surface Methodology and the optimal concentrations that have remarkably elevated carotenoid production were starch-2.24 g/l, MgSO 4 -0.416 g/l, ZnSO 4 -0.0157 g/l, and fumaric Acid-16 mM. Further, evaluation of pigment cytotoxicity against normal (Vero) and Non-Small Cell Carcinoma (A549) cells was performed. The resultant IC 50 values were quantified as 161.3 µg/ml and 7.623 µg/ml for Vero and A549 cells, respectively. Moreover, a reactive oxygen species (ROS) determination study in A549 cells was done which have shown a noteworthy threefold ROS production in A549 cells through fluorescence spectroscopic observation. This implies that the bacterial carotenoids can act as potent pro-oxidants against cancerous cells while being nontoxic toward normal cells.
فهرسة مساهمة: Keywords: Bacterial carotenoids; Plackett Burman design; cytotoxicity; reactive oxygen species; response surface methodology; tricarboxylic acid cycle intermediates
المشرفين على المادة: 36-88-4 (Carotenoids)
0 (Culture Media)
0 (Reactive Oxygen Species)
تواريخ الأحداث: Date Created: 20240102 Date Completed: 20240701 Latest Revision: 20240701
رمز التحديث: 20240701
DOI: 10.1080/10826068.2023.2282533
PMID: 38165781
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-2297
DOI:10.1080/10826068.2023.2282533