دورية أكاديمية
Optimization of media components for enhanced carotenoid production by Paracoccus marcusii RSPO1 and assessment of their cytotoxicity against A549 and vero cells.
العنوان: | Optimization of media components for enhanced carotenoid production by Paracoccus marcusii RSPO1 and assessment of their cytotoxicity against A549 and vero cells. |
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المؤلفون: | Naik R; Department of Microbiology, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Science (ARIBAS), CVM University, Anand, Gujarat, India., Gupte S; Department of Microbiology, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Science (ARIBAS), CVM University, Anand, Gujarat, India. |
المصدر: | Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2024 Jul; Vol. 54 (6), pp. 764-778. Date of Electronic Publication: 2024 Jan 02. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9607037 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2297 (Electronic) Linking ISSN: 10826068 NLM ISO Abbreviation: Prep Biochem Biotechnol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2005-> : Abingdon, Oxford : Taylor & Francis Original Publication: New York, NY : Marcel Dekker, c1996- |
مواضيع طبية MeSH: | Carotenoids*/pharmacology , Carotenoids*/metabolism , Paracoccus*/metabolism, Chlorocebus aethiops ; Animals ; Humans ; A549 Cells ; Vero Cells ; Culture Media/chemistry ; Reactive Oxygen Species/metabolism |
مستخلص: | In this study, we tried to explore the influence of various tricarboxylic acid (TCA) cycle intermediates on carotenoid production and with a focus on enhancing pigment biosynthesis, we conducted two statistical analysis. In case of TCA intermediates influence on pigment production by Paracoccus marcusii RSPO1; fumaric acid, and malic acid were observed as potent enhancers of pigment biosynthesis. Further, to optimize key media components for enhanced carotenoid production, the Plackett-Burman design was employed encompassing carbon, nitrogen sources, TCA cycle intermediates, and metal salts. The selected factors after Plackett Burman were fine-tuned through Response Surface Methodology and the optimal concentrations that have remarkably elevated carotenoid production were starch-2.24 g/l, MgSO |
فهرسة مساهمة: | Keywords: Bacterial carotenoids; Plackett Burman design; cytotoxicity; reactive oxygen species; response surface methodology; tricarboxylic acid cycle intermediates |
المشرفين على المادة: | 36-88-4 (Carotenoids) 0 (Culture Media) 0 (Reactive Oxygen Species) |
تواريخ الأحداث: | Date Created: 20240102 Date Completed: 20240701 Latest Revision: 20240701 |
رمز التحديث: | 20240701 |
DOI: | 10.1080/10826068.2023.2282533 |
PMID: | 38165781 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1532-2297 |
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DOI: | 10.1080/10826068.2023.2282533 |