دورية أكاديمية
أعرض في EDS

Low genetic diversity of Treponema pallidum ssp. pertenue (TPE) isolated from patients' ulcers in Namatanai District of Papua New Guinea: Local human population is infected by three TPE genotypes.

التفاصيل البيبلوغرافية
العنوان: Low genetic diversity of Treponema pallidum ssp. pertenue (TPE) isolated from patients' ulcers in Namatanai District of Papua New Guinea: Local human population is infected by three TPE genotypes.
المؤلفون: Medappa M; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Pospíšilová P; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Madruga MPM; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., John LN; National Department of Health, Aopi Centre, Port Moresby, Papua New Guinea.; Faculty of Medicine, University of Barcelona, Barcelona, Spain., Beiras CG; Faculty of Medicine, University of Barcelona, Barcelona, Spain., Grillová L; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Oppelt J; Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America., Banerjee A; Department of Mathematics and Statistics, Indian Institute of Technology, Kanpur, Uttar Pradesh, India., Vall-Mayans M; Skin NTDs and STI section, Fight Infectious Diseases Foundation, University Hospital Germans Trias i Pujol, Badalona, Spain., Mitjà O; Skin NTDs and STI section, Fight Infectious Diseases Foundation, University Hospital Germans Trias i Pujol, Badalona, Spain.; Lihir Medical Centre, Lihir Island, Papua New Guinea.; School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea., Šmajs D; Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 Jan 02; Vol. 18 (1), pp. e0011831. Date of Electronic Publication: 2024 Jan 02 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Treponema pallidum*/genetics , Yaws*/epidemiology, Child ; Humans ; Ulcer ; Multilocus Sequence Typing ; Papua New Guinea/epidemiology ; Treponema/genetics ; Mutation ; Genotype
مستخلص: Yaws is an endemic disease caused by Treponema pallidum subsp. pertenue (TPE) that primarily affects children in rural regions of the tropics. The endemic character of yaws infections and the expected exclusive reservoir of TPE in humans opened a new opportunity to start a yaws eradication campaign. We have developed a multi-locus sequence typing (MLST) scheme for TPE isolates combining the previously published (TP0548, TP0488) and new (TP0858) chromosomal loci, and we compared this typing scheme to the two previously published MLST schemes. We applied this scheme to TPE-containing clinical isolates obtained during a mass drug administration study performed in the Namatanai District of Papua New Guinea between June 2018 and December 2019. Of 1081 samples collected, 302 (28.5%) tested positive for TPE DNA, from which 255 (84.4%) were fully typed. The TPE PCR-positivity in swab samples was higher in younger patients, patients with single ulcers, first ulcer episodes, and with ulcer duration less than six months. Non-treponemal serological test positivity correlated better with PCR positivity compared to treponema-specific serological tests. The MLST revealed a low level of genetic diversity among infecting TPE isolates, represented by just three distinct genotypes (JE11, SE22, and TE13). Two previously used typing schemes revealed similar typing resolutions. Two new alleles (one in TP0858 and one in TP0136) were shown to arise by intragenomic recombination/deletion events. Compared to samples genotyped as JE11, the minor genotypes (TE13 and SE22) were more frequently detected in samples from patients with two or more ulcers and patients with higher values of specific TP serological tests. Moreover, the A2058G mutation in the 23S rRNA genes of three JE11 isolates was found, resulting in azithromycin resistance.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Medappa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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SCR Organism: Treponema pallidum subsp. pertenue
تواريخ الأحداث: Date Created: 20240102 Date Completed: 20240115 Latest Revision: 20240115
رمز التحديث: 20240115
مُعرف محوري في PubMed: PMC10786373
DOI: 10.1371/journal.pntd.0011831
PMID: 38166151
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0011831