دورية أكاديمية
أعرض في EDS

In Vitro Study on Four Types of Commercial Lectins on Leishmania infantum, L. major and L. tropica with Stage-Specific Binding and Leishmania Species Identification.

التفاصيل البيبلوغرافية
العنوان: In Vitro Study on Four Types of Commercial Lectins on Leishmania infantum, L. major and L. tropica with Stage-Specific Binding and Leishmania Species Identification.
المؤلفون: Haddadan SA; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Mohebali M; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.; Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran., Hajjaran H; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Foroushani AR; Department of Epidemiology and Biostatics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Kakooei Z; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Afshar MJA; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.; School of Allied Medical Sciences, Jiroft University of Medical Sciences, Jiroft, Iran., Zarei Z; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Alizadeh Z; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Akhoundi B; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
المصدر: Iranian journal of parasitology [Iran J Parasitol] 2023 Oct-Dec; Vol. 18 (4), pp. 456-463.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Tehran University of Medical Sciences Country of Publication: Iran NLM ID: 101464309 Publication Model: Print Cited Medium: Print ISSN: 1735-7020 (Print) Linking ISSN: 17357020 NLM ISO Abbreviation: Iran J Parasitol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Tehran : Tehran University of Medical Sciences
مستخلص: Background: We aimed to verify the susceptibility of Leishmania infantum , L. major and L. tropica , to commercial lectins in order to identify the three Leishmania species.
Methods: The degree of agglutination was determined both macroscopically and microscopically and was scored negative (-) to positive (from 1 + - 4 + ) based on their percentage of agglutination.
Results: Jacalin and UEA-1 were capable of agglutination of L. infantum isolates in both logarithmic and stationary phases at a concentration of 1000 μg/ml (100%). L. tropica isolates showed agglutination with the lectin UEA-1 in both logarithmic and stationary phases (62.5% and 87.5%). L. major and L. tropica showed 75% agglutination with lectin Jacalin in both logarithmic and stationary phases. L. tropica isolates showed 25% agglutination with the lectin WGA in the logarithmic phase. L. infantum , L. major and L. tropica isolates showed 25, 12.5 and 37.5% agglutination in the stationary phase, however, did not show agglutination in logarithmic phases. L. major isolates showed 12.5% agglutination with the lectin PHA in the stationary phase, however, were incapable of agglutination with the L. tropica and L. infantum in both logarithmic and stationary phases.
Conclusion: Despite the fact, that JCA and I-UEA lectins were not able to completely separate L. infantum , L. major and L. tropica . WGA lectin and PHA lectin can help in separating the species of Leishmania parasites.
Competing Interests: Conflicts of interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.
(© 2023 Aghaee Haddadan et al. Published by Tehran University of Medical Sciences.)
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فهرسة مساهمة: Keywords: Agglutination; In vitro; Lectin; Leishmania; Species identification
تواريخ الأحداث: Date Created: 20240103 Latest Revision: 20240105
رمز التحديث: 20240105
مُعرف محوري في PubMed: PMC10758074
DOI: 10.18502/ijpa.v18i4.14245
PMID: 38169603
قاعدة البيانات: MEDLINE
الوصف
تدمد:1735-7020
DOI:10.18502/ijpa.v18i4.14245