دورية أكاديمية
Suspension culture of stem cells established of Calendula officinalis L.
| العنوان: | Suspension culture of stem cells established of Calendula officinalis L. |
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| المؤلفون: | Kaňuková Š; Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 917 01, Trnava, Slovakia.; Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 921 68, Piešťany, Slovakia., Lenkavská K; Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 917 01, Trnava, Slovakia., Gubišová M; Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 921 68, Piešťany, Slovakia., Kraic J; Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 917 01, Trnava, Slovakia. jan.kraic@ucm.sk.; Research Institute of Plant Production, National Agricultural and Food Center, Bratislavská cesta 122, 921 68, Piešťany, Slovakia. jan.kraic@ucm.sk. |
| المصدر: | Scientific reports [Sci Rep] 2024 Jan 03; Vol. 14 (1), pp. 441. Date of Electronic Publication: 2024 Jan 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Calendula* , Plants, Medicinal*, Cell Culture Techniques/methods ; Plant Leaves ; Stem Cells |
| مستخلص: | Plant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or stem cell-like cells in other organs and tissues of the plant body, as well as the possibility of de novo generation of meristematic cells from differentiated cells, allow to consider the establishment of stem cell cultures in a broader range of species. This study aimed to establish a stem cell culture of the medicinal plant Calendula officinalis L. Callus tissues were induced from leaf and root explants, and already at this stage, stem and dedifferentiated cells could be identified. The cell suspension cultures established both from the root- and leaf-derived calli contained a high proportion of stem cells (92-93% and 72-73%, respectively). The most effective combination of growth regulators for the development of stem cells in calli as well as cell cultures was 1.0 mg/L 2,4-D and 0.5 mg/L BAP. The highest amount of stem cells (5.60-5.72 × 10 5 ) was in cell suspension derived from the roots. An effective protocol for the establishment of marigold stem cell suspension culture was developed. The ratio of root-derived stem cells against dedifferentiated cells exceeded 90%. (© 2024. The Author(s).) |
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| تواريخ الأحداث: | Date Created: 20240103 Date Completed: 20240105 Latest Revision: 20240108 |
| رمز التحديث: | 20240108 |
| مُعرف محوري في PubMed: | PMC10764935 |
| DOI: | 10.1038/s41598-023-50945-0 |
| PMID: | 38172230 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-023-50945-0 |