دورية أكاديمية
Viperin mutation is linked to immunity, immune cell dynamics, and metabolic alteration during VHSV infection in zebrafish.
| العنوان: | Viperin mutation is linked to immunity, immune cell dynamics, and metabolic alteration during VHSV infection in zebrafish. |
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| المؤلفون: | Shanaka KASN; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea.; Marine Science Institute, Jeju National University, Jeju, Republic of Korea., Jung S; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea.; Marine Science Institute, Jeju National University, Jeju, Republic of Korea., Madushani KP; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea., Kim MJ; Bioresource Industrialization Center, Nakdonggang National Institute of Biological Resources, Sangju-si, Gyeongsangbuk-do, Republic of Korea., Lee J; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea.; Marine Science Institute, Jeju National University, Jeju, Republic of Korea. |
| المصدر: | Frontiers in immunology [Front Immunol] 2023 Dec 19; Vol. 14, pp. 1327749. Date of Electronic Publication: 2023 Dec 19 (Print Publication: 2023). |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Hemorrhagic Septicemia, Viral* , Perciformes*, Animals ; Cholesterol ; Mammals ; Proteins ; Zebrafish ; Viperin Protein/metabolism ; Zebrafish Proteins/metabolism |
| مستخلص: | Viperin is a prominent antiviral protein found in animals. The primary function of Viperin is the production of 3'-deoxy-3',4'-didehydro-cytidine triphosphate (ddhCTP), an inhibitory nucleotide involved in viral RNA synthesis. Studies in mammalian models have suggested that ddhCTP interferes with metabolic proteins. However, this hypothesis has yet to be tested in teleost. In this study, the role of Viperin in regulating metabolic alterations during viral hemorrhagic septicemia virus (VHSV) infection was tested. When infected with VHSV, viperin -/- fish showed considerably higher mortality rates. VHSV copy number and the expression of the NP gene were significantly increased in viperin -/- fish. Metabolic gene analysis revealed significant differences in soda , hif1a , fasn , and acc expression, indicating their impact on metabolism. Cholesterol analysis in zebrafish larvae during VHSV infection showed significant upregulation of cholesterol production without Viperin. In vitro analysis of ZF4 cells suggested a considerable reduction in lipid production and a significant upregulation of reactive oxygen species (ROS) generation with the overexpression of viperin . Neutrophil and macrophage recruitment were significantly modulated in viperin -/- fish compared to the wild-type (WT) fish. Thus, we have demonstrated that Viperin plays a role in interfering with metabolic alterations during VHSV infection. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Shanaka, Jung, Madushani, Kim and Lee.) |
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| فهرسة مساهمة: | Keywords: RNA virus; VHSV; Viperin; antiviral protein; zebrafish |
| المشرفين على المادة: | 97C5T2UQ7J (Cholesterol) 0 (Proteins) 0 (Viperin Protein) 0 (Zebrafish Proteins) |
| تواريخ الأحداث: | Date Created: 20240104 Date Completed: 20240108 Latest Revision: 20240405 |
| رمز التحديث: | 20240406 |
| مُعرف محوري في PubMed: | PMC10763233 |
| DOI: | 10.3389/fimmu.2023.1327749 |
| PMID: | 38173722 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2023.1327749 |