دورية أكاديمية
Viperin mutation is linked to immunity, immune cell dynamics, and metabolic alteration during VHSV infection in zebrafish.
العنوان: | Viperin mutation is linked to immunity, immune cell dynamics, and metabolic alteration during VHSV infection in zebrafish. |
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المؤلفون: | Shanaka KASN; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea.; Marine Science Institute, Jeju National University, Jeju, Republic of Korea., Jung S; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea.; Marine Science Institute, Jeju National University, Jeju, Republic of Korea., Madushani KP; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea., Kim MJ; Bioresource Industrialization Center, Nakdonggang National Institute of Biological Resources, Sangju-si, Gyeongsangbuk-do, Republic of Korea., Lee J; Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju, Republic of Korea.; Marine Science Institute, Jeju National University, Jeju, Republic of Korea. |
المصدر: | Frontiers in immunology [Front Immunol] 2023 Dec 19; Vol. 14, pp. 1327749. Date of Electronic Publication: 2023 Dec 19 (Print Publication: 2023). |
نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
مواضيع طبية MeSH: | Hemorrhagic Septicemia, Viral* , Perciformes*, Animals ; Cholesterol ; Mammals ; Proteins ; Zebrafish ; Viperin Protein/metabolism ; Zebrafish Proteins/metabolism |
مستخلص: | Viperin is a prominent antiviral protein found in animals. The primary function of Viperin is the production of 3'-deoxy-3',4'-didehydro-cytidine triphosphate (ddhCTP), an inhibitory nucleotide involved in viral RNA synthesis. Studies in mammalian models have suggested that ddhCTP interferes with metabolic proteins. However, this hypothesis has yet to be tested in teleost. In this study, the role of Viperin in regulating metabolic alterations during viral hemorrhagic septicemia virus (VHSV) infection was tested. When infected with VHSV, viperin -/- fish showed considerably higher mortality rates. VHSV copy number and the expression of the NP gene were significantly increased in viperin -/- fish. Metabolic gene analysis revealed significant differences in soda , hif1a , fasn , and acc expression, indicating their impact on metabolism. Cholesterol analysis in zebrafish larvae during VHSV infection showed significant upregulation of cholesterol production without Viperin. In vitro analysis of ZF4 cells suggested a considerable reduction in lipid production and a significant upregulation of reactive oxygen species (ROS) generation with the overexpression of viperin . Neutrophil and macrophage recruitment were significantly modulated in viperin -/- fish compared to the wild-type (WT) fish. Thus, we have demonstrated that Viperin plays a role in interfering with metabolic alterations during VHSV infection. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Shanaka, Jung, Madushani, Kim and Lee.) |
References: | Viruses. 2014 May 23;6(5):2204-13. (PMID: 24859343) Front Physiol. 2018 May 01;9:419. (PMID: 29765329) Nat Metab. 2022 Oct;4(10):1245-1259. (PMID: 36266542) BMB Rep. 2019 Jun;52(6):360-372. (PMID: 31186085) Chem Phys Lipids. 2016 Sep;199:136-143. (PMID: 27179407) PLoS Pathog. 2013;9(8):e1003497. (PMID: 23935494) EMBO J. 2018 Oct 15;37(20):. (PMID: 30209241) FEBS Lett. 2020 May;594(10):1631-1644. (PMID: 32232843) Int J Mol Sci. 2022 Jul 27;23(15):. (PMID: 35955436) J Immunol. 2010 May 15;184(10):5723-31. (PMID: 20410488) Front Immunol. 2021 Feb 24;12:581786. (PMID: 33717065) J Vis Exp. 2012 Nov 18;(69):e4196. (PMID: 23183629) Annu Rev Virol. 2021 Sep 29;8(1):373-391. (PMID: 34586876) Fish Shellfish Immunol. 2019 Apr;87:565-572. (PMID: 30742890) J Nutr. 2021 Oct 1;151(10):2986-2996. (PMID: 34383941) Front Microbiol. 2020 Oct 20;11:574231. (PMID: 33193184) J Leukoc Biol. 2010 Jan;87(1):93-106. (PMID: 20052802) Clin Chem. 2009 Apr;55(4):611-22. (PMID: 19246619) Front Immunol. 2015 Apr 08;6:164. (PMID: 25904920) Fish Shellfish Immunol. 2022 Dec;131:672-681. (PMID: 36309322) Dis Aquat Organ. 2011 Nov 3;97(1):25-35. (PMID: 22235592) Nat Cell Biol. 2019 Jan;21(1):85-93. (PMID: 30602764) Science. 2009 May 22;324(5930):1029-33. (PMID: 19460998) ACS Bio Med Chem Au. 2023 Jul 25;3(4):322-326. (PMID: 37599790) Arterioscler Thromb Vasc Biol. 2007 Apr;27(4):755-61. (PMID: 17272744) J Biol Chem. 2021 Jul;297(1):100824. (PMID: 34029588) Curr Opin Virol. 2018 Feb;28:37-42. (PMID: 29156318) Front Immunol. 2022 Apr 21;13:791267. (PMID: 35529872) Front Immunol. 2021 Feb 03;11:594963. (PMID: 33613518) Fish Shellfish Immunol. 2023 Oct;141:109006. (PMID: 37598733) Clin Rev Allergy Immunol. 2021 Oct;61(2):101-113. (PMID: 32524254) Front Physiol. 2021 Oct 07;12:749770. (PMID: 34690817) Immune Netw. 2018 Aug 23;18(4):e32. (PMID: 30181920) Annu Rev Virol. 2020 Sep 29;7(1):421-446. (PMID: 32603630) Nat Metab. 2021 Nov;3(11):1466-1475. (PMID: 34580494) J Virol. 2021 Aug 10;95(17):e0078121. (PMID: 34132567) |
فهرسة مساهمة: | Keywords: RNA virus; VHSV; Viperin; antiviral protein; zebrafish |
المشرفين على المادة: | 97C5T2UQ7J (Cholesterol) 0 (Proteins) 0 (Viperin Protein) 0 (Zebrafish Proteins) |
تواريخ الأحداث: | Date Created: 20240104 Date Completed: 20240108 Latest Revision: 20240405 |
رمز التحديث: | 20240406 |
مُعرف محوري في PubMed: | PMC10763233 |
DOI: | 10.3389/fimmu.2023.1327749 |
PMID: | 38173722 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-3224 |
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DOI: | 10.3389/fimmu.2023.1327749 |