دورية أكاديمية
Changes in the saliva proteome analysed by gel-proteomics in horses diagnosed with equine gastric ulcer syndrome (EGUS) at diagnosis and after successful treatment.
| العنوان: | Changes in the saliva proteome analysed by gel-proteomics in horses diagnosed with equine gastric ulcer syndrome (EGUS) at diagnosis and after successful treatment. |
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| المؤلفون: | López-Martínez MJ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain. Electronic address: mariajose.lopez28@um.es., Lamy E; MED - Mediterranean Institute for Agriculture, Environment and Development & CHANGE - Global Change and Sustainability Institute, University of Évora, Évora, Portugal. Electronic address: ecsl@uevora.pt., Cerón JJ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain. Electronic address: jjceron@um.es., Ayala I; Department of Animal Medicine & Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain. Electronic address: iayape@um.es., Contreras-Aguilar MD; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain. Electronic address: mariadolores.contreras@um.es., Henriksen IH; Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark. Electronic address: ida-marie.boll@sund.ku.dk., Muñoz-Prieto A; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain. Electronic address: alberto.munoz@um.es., Hansen S; Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark. Electronic address: sannih@sund.ku.dk. |
| المصدر: | Research in veterinary science [Res Vet Sci] 2024 Feb; Vol. 167, pp. 105112. Date of Electronic Publication: 2023 Dec 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : British Veterinary Association Original Publication: Oxford. |
| مواضيع طبية MeSH: | Stomach Ulcer*/diagnosis , Stomach Ulcer*/veterinary , Stomach Ulcer*/pathology , Horse Diseases*/pathology, Animals ; Horses ; Proteome ; Proteomics ; Saliva |
| مستخلص: | Equine gastric ulcer syndrome (EGUS) is currently one of the more frequent diseases in horses. We aimed to identify changes in the salivary proteome in horses with EGUS at diagnosis and after successful treatment by using gel proteomics. Saliva samples were collected from nine horses with EGUS before and after treatment and nine matched healthy controls. SDS-PAGE (1DE) and two-dimensional gel electrophoresis (2DE) were performed, and significantly different protein bands and spots were identified by mass spectrometry. Horses with EGUS had increases in proteins such as adenosine deaminase (ADA), triosephosphate isomerase, keratins and immuno-globulin heavy constant mu and decreases in carbonic anhydrase (CA), albumin and prolactin-induced protein. These changes would indicate various physiopathological mechanisms involved in this disease, such as the activation of the immune system, decreased stomach defence mechanisms and inflammation. The treated horses presented lower expression levels of thioredoxin (TRX) after a successful treatment, in proteomics analysis and also measured with a commercially available ELISA kit. Overall, horses with EGUS have protein changes in their saliva when measured with gel proteomics compared with healthy horses, and they also showed changes after successful treatment. These proteins could be potential biomarkers for detection and monitoring treatment response in EGUS. Competing Interests: Declaration of Competing Interest The authors of this manuscript have no financial or personal relationships that could potentially influence the content and findings presented in the manuscript. We confirm that there are no affiliations with organizations or entities that have a direct interest in the subject matter discussed in the article. Our commitment to transparency and integrity in research is paramount, and we assure you that the research was conducted objectively and without any external influences that could compromise the validity of the results. We adhere to the highest ethical standards in the preparation and submission of this manuscript to Research in Veterinary Science. (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biomarkers; EGUS; Horses; Salivary proteome |
| المشرفين على المادة: | 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20240104 Date Completed: 20240116 Latest Revision: 20240116 |
| رمز التحديث: | 20240116 |
| DOI: | 10.1016/j.rvsc.2023.105112 |
| PMID: | 38176208 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2661 |
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| DOI: | 10.1016/j.rvsc.2023.105112 |